Multicenter Clinical Evaluation of the Automated Aries Group A Strep PCR Assay from Throat Swabs
| Autor: | Neena Kanwar, Ronald Dunn, C. Ulen, Timothy S. Uphoff, Jordan Crawford, A. Drain, Rangaraj Selvarangan, J. Dien Bard |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male 0301 basic medicine Microbiology (medical) medicine.medical_specialty Time Factors Adolescent Streptococcus pyogenes 030106 microbiology medicine.disease_cause Polymerase Chain Reaction Sensitivity and Specificity Gastroenterology Young Adult 03 medical and health sciences 0302 clinical medicine Streptococcal Infections Throat Internal medicine medicine Humans Prospective Studies 030212 general & internal medicine Child Prospective cohort study Aged Aged 80 and over Automation Laboratory Streptococcus business.industry Infant Bacteriology Assay sensitivity Middle Aged United States Confidence interval Pharyngitis respiratory tract diseases medicine.anatomical_structure Molecular Diagnostic Techniques Child Preschool Pharynx Female Sample collection medicine.symptom business |
| Zdroj: | Journal of Clinical Microbiology. 57 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.01482-18 |
| Popis: | Group A Streptococcus (GAS) is one of the leading causes of bacterial pharyngitis. Early GAS diagnosis is critical for appropriate antibiotic administration that reduces the risk of GAS sequelae and limits spread of the infection. The Aries Group A Strep (GAS) assay (Luminex, Austin, TX) is a fully automated PCR assay for direct detection of GAS in throat swab specimens in less than 2 h with minimum hands-on time. This multicenter prospective study evaluated the clinical performance of the Aries GAS assay compared to that of Streptococcus pyogenes culture. Subjects with symptoms consistent with pharyngitis were enrolled across four sites in the United States, and a throat swab in liquid Amies medium was obtained. Aries and reference testing was performed within 72 and 48 h after sample collection, respectively. Of 623 throat swab specimens from patients with pharyngitis (93.6% |
| Databáze: | OpenAIRE |
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