Reducing the Oxidation Level of Dextran Aldehyde in a Chitosan/Dextran-Based Surgical Hydrogel Increases Biocompatibility and Decreases Antimicrobial Efficacy
| Autor: | Jaydee D. Cabral, Stephen C. Moratti, Maggie Chan, Heather J. L. Brooks, Lyall R. Hanton |
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| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
Biocompatibility Biocompatible Materials Pharmacology Article Catalysis Cell Line lcsh:Chemistry Inorganic Chemistry Chitosan chemistry.chemical_compound biocompatibility Anti-Infective Agents In vivo medicine Humans oxidized dextran Physical and Theoretical Chemistry lcsh:QH301-705.5 Molecular Biology Spectroscopy Aldehydes Organic Chemistry technology industry and agriculture Dextrans Hydrogels General Medicine Adhesion Fibroblasts Antimicrobial Computer Science Applications Surgery Dextran lcsh:Biology (General) lcsh:QD1-999 chemistry Self-healing hydrogels antimicrobial hydrogel Wound healing Oxidation-Reduction |
| Zdroj: | International Journal of Molecular Sciences Volume 16 Issue 6 Pages 13798-13814 International Journal of Molecular Sciences, Vol 16, Iss 6, Pp 13798-13814 (2015) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms160613798 |
| Popis: | A highly oxidized form of a chitosan/dextran-based hydrogel (CD-100) containing 80% oxidized dextran aldehyde (DA-100) was developed as a post-operative aid, and found to significantly prevent adhesion formation in endoscopic sinus surgery (ESS). However, the CD-100 hydrogel showed moderate in vitro cytotoxicity to mammalian cell lines, with the DA-100 found to be the cytotoxic component. In order to extend the use of the hydrogel to abdominal surgeries, reformulation using a lower oxidized DA (DA-25) was pursued. The aim of the present study was to compare the antimicrobial efficacy, in vitro biocompatibility and wound healing capacity of the highly oxidized CD-100 hydrogel with the CD-25 hydrogel. Antimicrobial studies were performed against a range of clinically relevant abdominal microorganisms using the micro-broth dilution method. Biocompatibility testing using human dermal fibroblasts was assessed via a tetrazolium reduction assay (MTT) and a wound healing model. In contrast to the original DA-100 formulation, DA-25 was found to be non-cytotoxic, and showed no overall impairment of cell migration, with wound closure occurring at 72 h. However, the lower oxidation level negatively affected the antimicrobial efficacy of the hydrogel (CD-25). Although the CD-25 hydrogel’s antimicrobial efficacy and anti-fibroblast activity is decreased when compared to the original CD-100 hydrogel formulation, previous in vivo studies show that the CD-25 hydrogel remains an effective, biocompatible barrier agent in the prevention of postoperative adhesions. |
| Databáze: | OpenAIRE |
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