Effects of TGF- 2 on mineral resorption in cultured embryonic mouse long bones; 45Ca release and osteoclast differentiation and migration
| Autor: | S C Dieudonné, H M Berghuis, W Goei, J P Veldhuijzen |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
medicine.medical_specialty
Acid Phosphatase Long bone Osteoclasts Orthodontics Bone and Bones Bone resorption Mice Cell Movement Transforming Growth Factor beta Osteoclast Internal medicine medicine Animals Bone Resorption Tartrate-resistant acid phosphatase Periosteum biology Chemistry Acid phosphatase Cell Differentiation Resorption medicine.anatomical_structure Endocrinology biology.protein Calcium Metatarsal bones |
| Zdroj: | The European Journal of Orthodontics. 16:130-137 |
| ISSN: | 1460-2210 0141-5387 |
| DOI: | 10.1093/ejo/16.2.130 |
| Popis: | To study the effects of transforming growth factor beta 2 (TGF-beta 2) on bone resorption, cultures of 17-day-old foetal mouse metatarsal long bones were used. The long bone rudiments were cultured for 5 days in medium supplemented with 10% rat serum. The effects of TGF-beta 2 were studied at concentrations of 1, 4, and 10 ng/ml. At all concentrations TGF-beta 2 caused a significant reduction in osteoclastic resorption measured as release of 45Ca from prelabelled bones. The same long bones were subsequently used for histological evaluations. Pre-osteoclasts and osteoclasts were identified as tartrate resistant acid phosphatase (TRAP) positive cells in the mineralized diaphysis, the periosteum around the diaphysis, and the perichondrium around the cartilaginous ends. The distribution of TRAP-positive cells over the three compartments showed that TGF-beta 2 inhibited the migration of TRAP-positive cells from the periosteum into the mineralized diaphysis in a dose dependent manner. In addition, TGF-beta 2 had a biphasic effect on TRAP cell differentiation, as 1 ng/ml increased, but 4 ng/ml and higher decreased TRAP cell numbers. We conclude that TGF-beta 2 is a potent regulator of osteoclastic bone resorption, by modulating both osteoclast migration and osteoclast differentiation. |
| Databáze: | OpenAIRE |
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