Enrichment and acclimation of an anaerobic mesophilic microorganism's inoculum for standardization of BMP assays
| Autor: | Hugo Moreira Soares, Melissa Paola Mezzari, Deisi Cristina Tápparo, Marcio Luis Busi da Silva, Ricardo Luis Radis Steinmetz, André Cestonaro do Amaral, Airton Kunz |
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| Přispěvatelé: | RICARDO LUIS RADIS STEINMETZ, CNPSA, MELISSA PAOLA MEZZARI, UNOESC, MARCIO LUIS BUSI DA SILVA, CNPSA, AIRTON KUNZ, CNPSA, ANDRÉ CESTONARO DO AMARAL, UNIOESTE, DEISI CRISTINA TÁPPARO, UNIOESTE, HUGO MOREIRA SOARES, UFSC. |
| Rok vydání: | 2016 |
| Předmět: |
Dejeto
Environmental Engineering 020209 energy Microorganism Acclimatization Biogás Bioengineering Methanobacteriales 02 engineering and technology 010501 environmental sciences Biology Alkalies Real-Time Polymerase Chain Reaction 01 natural sciences DNA Ribosomal Microbiology Bioreactors Biogas Anaerobic digestion 0202 electrical engineering electronic engineering information engineering Food science Anaerobiosis Cellulose Waste Management and Disposal 0105 earth and related environmental sciences Renewable Energy Sustainability and the Environment Temperature General Medicine Reference Standards biology.organism_classification Digestão anaeróbica Fatty Acids Volatile Methanogen Archaea Waste treatment Biofuels Methanosarcinales Methanomicrobiales Methane Mesophile |
| Zdroj: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
| ISSN: | 1873-2976 |
| Popis: | Appropriate enrichment of anaerobic microorganism?s consortium is crucial for accurate biochemical methane potential (BMP) assays. An alternative method to produce and maintain a mesophilic methanogenic inoculum was demonstrated. Three sources of inoculum were mixed and acclimated for 857 days in order to reach steady conditions (pH = 7.90 ± 0.46; VS/TS > 50%; VFA/alkalinity = 0.16 ± 0.04 gAcetic Acid/gCaCO3 ). Biogas yield >80% was obtained after 70 days of inoculum acclimation in comparison to standard cellulose (>600 mLN/gVS). Methanogen community analysis based on 16S rDNA of the inoculum revealed Archaea concentration of 3 1012 gene copies/g (Methanobacteriales 8 1010; Methanomicrobiales 8 1010; and Methanosarcinales 4 1011 gene copies/g). The proposed method for development and maintenance of microorganism enrichment inoculum demonstrates consistent BMP data which is a requirement for dependable prediction of biogas production at field scale operations. Made available in DSpace on 2019-07-19T00:40:37Z (GMT). No. of bitstreams: 1 final8317.pdf: 419817 bytes, checksum: ae579155ca7e4e55877f1e5682cd35c3 (MD5) Previous issue date: 2016 |
| Databáze: | OpenAIRE |
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