Normobaric oxygen treatment improves neuronal survival functional recovery and axonal plasticity after newborn hypoxia-ischemia
| Autor: | Serdar Altunay, Selim Kutlu, Taha Kelestemur, Mustafa Çağlar Beker, Ertugrul Kilic, Berrak Caglayan, Ahmet Burak Çağlayan |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
medicine.medical_specialty
PTEN Plasticity Cell Survival Spatial Learning Motor Activity Open field Infant Newborn Diseases Rats Sprague-Dawley 03 medical and health sciences Behavioral Neuroscience Epilepsy 0302 clinical medicine Internal medicine Functional Recovery medicine Animals Humans 030304 developmental biology Hyperoxia Neurons 0303 health sciences Neuronal Plasticity biology Behavior Animal business.industry Infant Newborn Oxygen Inhalation Therapy PTEN Phosphohydrolase Recovery of Function medicine.disease Normobaric Oxygen Barnes maze Motor coordination Newborn Hypoxia Ischemia Rats Disease Models Animal Endocrinology Animals Newborn Matrix Metalloproteinase 9 Apoptosis Hypoxia-Ischemia Brain biology.protein medicine.symptom business MMP-9 030217 neurology & neurosurgery |
| Zdroj: | Behavioural brain research. 379 |
| ISSN: | 1872-7549 |
| Popis: | Background: Newborn hypoxia ischemia (HI) is one of the most prevalent cases in the emergency and can result from fetal hypoxia during delivery. In HI, restricted blood supply to the fetal brain may cause epilepsy or mental disorders.Methods: In the present study, seven-day-old pups were subjected HI and treated with different normobaric oxygen (NBO) concentrations (21%, 70% or 100%). In the acute phase, we analyzed infarct area, disseminate neuronal injury and surviving neurons. In addition, we studied the regulation of PTEN and MMP-9 proteins which were suggested to be activated by HI in the ischemic tissue. Moreover, long-term effects of NBO treatments were evaluated with open field, rotarod and Barnes maze tests. We also examined axonal plasticity with EGFP-AAV injection.Results: Here, we demonstrate that hyperoxic NBO concentration causes an increase in cellular survival and a decrease in the number of apoptotic cells, meanwhile inhibiting the proteins involved in cellular death mechanisms. Moreover, we found that hyperoxia decreases anxiety, promotes motor coordination and improve spatial learning and memory. Notably that axonal sprouting was promoted by hyperoxia.Conclusion: Our data suggest that NBO is a promising approach for the treatment of newborn HI, which encourage proof-of-concept studies in newborn. Turkish Academy of Sciences Necmettin Erbakan University |
| Databáze: | OpenAIRE |
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