Propionibacterium acnes biofilm is present in intervertebral discs of patients undergoing microdiscectomy
| Autor: | Radim Lipina, Garth A. James, Ondrej Slaby, John C. Baird, Jiri Sana, Nitin N. Bhatia, Christof Birkenmaier, Jeffrey C. Wang, A. Nick Shamie, Assaf Raz, Robert K. Eastlack, Jerry Stonemetz, Filip Ruzicka, Steven R. Garfin, Neel Anand, Elleni Michu, Jonathan E. Schmitz, Philip S. Stewart, Radim Jančálek, Jaspaul S. Gogia, Vincent A. Fischetti, Tana Machackova, Konstantinos Mavrommatis, Michael F. Coscia, Fahad S. Ahmed, Steve T. Fisher, Hana Nosková, Timothy F. Witham, Yu-Po Lee, Manu N. Capoor, Ziya L. Gokaslan, Martin Smrčka, Calvin C. Kuo, Todd Alamin, Sibel Demir-Deviren |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
SAPHO syndrome
Male Pathology Confocal Microscopy Staphylococcus complications microbiology [Gram-Positive Bacterial Infections] lcsh:Medicine Intervertebral Disc Degeneration medicine.disease_cause Pathology and Laboratory Medicine Mass Spectrometry Analytical Chemistry 0302 clinical medicine Spectrum Analysis Techniques Fluorescence Microscopy Medicine and Health Sciences 030212 general & internal medicine Scanning Confocal Microscopy Intervertebral Disc Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry lcsh:Science etiology microbiology surgery [Intervertebral Disc Displacement] Aged 80 and over Microscopy Multidisciplinary biology Light Microscopy Middle Aged Bacterial Pathogens growth & development [Biofilms] Chemistry medicine.anatomical_structure Phenotype Medical Microbiology isolation & purification pathogenicity physiology [Propionibacterium acnes] Physical Sciences Staphylococcus haemolyticus Female Pathogens Intervertebral Disc Displacement Diskectomy Research Article Adult medicine.medical_specialty Surgical and Invasive Medical Procedures Research and Analysis Methods Microbiology 03 medical and health sciences Propionibacterium acnes Young Adult Multiplex polymerase chain reaction medicine Humans Microbial Pathogens Gram-Positive Bacterial Infections surgery [Intervertebral Disc] Aged Bacteria lcsh:R Biofilm Organisms Biology and Life Sciences Intervertebral disc Bacteriology medicine.disease biology.organism_classification microbiology [Intervertebral Disc Degeneration] Biofilms Staphylococcus Haemolyticus lcsh:Q Bacterial Biofilms Confocal Laser Microscopy 030217 neurology & neurosurgery |
| Zdroj: | PLoS ONE, Vol 12, Iss 4, p e0174518 (2017) PLoS ONE Capoor, Manu N; Ruzicka, Filip; Schmitz, Jonathan E; James, Garth A; Machackova, Tana; Jancalek, Radim; et al.(2017). Propionibacterium acnes biofilm is present in intervertebral discs of patients undergoing microdiscectomy.. PloS one, 12(4), e0174518-e01745e0174518. UC Irvine: Institute for Clinical and Translational Science. Retrieved from: http://www.escholarship.org/uc/item/8qn97751 |
| ISSN: | 1932-6203 |
| Popis: | Background In previous studies, Propionibacterium acnes was cultured from intervertebral disc tissue of similar to 25% of patients undergoing microdiscectomy, suggesting a possible link between chronic bacterial infection and disc degeneration. However, given the prominence of P. acnes as a skin commensal, such analyses often struggled to exclude the alternate possibility that these organisms represent perioperative microbiologic contamination. This investigation seeks to validate P. acnes prevalence in resected disc cultures, while providing microscopic evidence of P. acnes biofilm in the intervertebral discs. Methods Specimens from 368 patients undergoing microdiscectomy for disc herniation were divided into several fragments, one being homogenized, subjected to quantitative anaerobic culture, and assessed for bacterial growth, and a second fragment frozen for additional analyses. Colonies were identified by MALDI-TOF mass spectrometry and P. acnes phylotyping was conducted by multiplex PCR. For a sub-set of specimens, bacteria localization within the disc was assessed by microscopy using confocal laser scanning and FISH. Results Bacteria were cultured from 162 discs (44%), including 119 cases (32.3%) with P. acnes. In 89 cases, P. acnes was cultured exclusively; in 30 cases, it was isolated in combination with other bacteria (primarily coagulase-negative Staphylococcus spp.) Among positive specimens, the median P. acnes bacterial burden was 350 CFU/g (12 - similar to 20,000 CFU/g). Thirtyeight P. acnes isolates were subjected to molecular sub-typing, identifying 4 of 6 defined phylogroups: IA1, IB, IC, and II. Eight culture-positive specimens were evaluated by fluorescence microscopy and revealed P. acnes in situ. Notably, these bacteria demonstrated a biofilm distribution within the disc matrix. P. acnes bacteria were more prevalent in males than females (39% vs. 23%, p = 0.0013). Conclusions This study confirms that P. acnes is prevalent in herniated disc tissue. Moreover, it provides the first visual evidence of P. acnes biofilms within such specimens, consistent with infection rather than microbiologic contamination. |
| Databáze: | OpenAIRE |
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