In vitro effects of two silicate-based materials, Biodentine and BioRoot RCS, on dental pulp stem cells in models of reactionary and reparative dentinogenesis

Autor: Benjamin Fournier, Stéphane Simon, Ludwig Stanislas Loison-Robert, Eric Bonte, Tsouria Berbar, Mathilde Tassin, Ariane Berdal, Juliane Isaac
Přispěvatelé: Université Paris Descartes - Faculté de Chirurgie Dentaire (UPD5 Odontologie), Université Paris Descartes - Paris 5 (UPD5), Centre de Recherche des Cordeliers (CRC), Université Pierre et Marie Curie - Paris 6 (UPMC)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris Diderot - Paris 7 - UFR Odontologie (UPD7 Odontologie), Université Paris Diderot - Paris 7 (UPD7), HAL UPMC, Gestionnaire
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Biomineralization
Teeth
Physiology
Cellular differentiation
lcsh:Medicine
Biocompatible Materials
Apoptosis
Mineralization (biology)
Extracellular matrix
chemistry.chemical_compound
0302 clinical medicine
Dentin
Medicine and Health Sciences
Enzyme assays
Colorimetric assays
lcsh:Science
Bioassays and physiological analysis
Cytoskeleton
Staining
Multidisciplinary
MTT assay
Cell Death
Stem Cells
Cell Staining
Cell Differentiation
Hydrogen-Ion Concentration
Endodontics
Extracellular Matrix
medicine.anatomical_structure
Cell Processes
Calcium silicate
Physical Sciences
Cements
Stem cell
Anatomy
Research Article
Biotechnology
medicine.medical_specialty
Materials Science
In Vitro Techniques
Real-Time Polymerase Chain Reaction
Research and Analysis Methods
Models
Biological
Biomaterials
03 medical and health sciences
Dental Materials
stomatognathic system
Dental pulp stem cells
medicine
Binders
Humans
RNA
Messenger
[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/Biomaterials
Dental Pulp
Materials by Attribute
Cell Proliferation
Silicates
lcsh:R
Biology and Life Sciences
030206 dentistry
Cell Biology
[SDV.IB.BIO] Life Sciences [q-bio]/Bioengineering/Biomaterials
Oxidative Stress
030104 developmental biology
chemistry
Jaw
Specimen Preparation and Treatment
Biochemical analysis
Biophysics
Microscopy
Electron
Scanning
lcsh:Q
Physiological Processes
Digestive System
Head
Developmental Biology
Zdroj: PLoS ONE
PLoS ONE, Public Library of Science, 2018, 13 (1), pp.e0190014. ⟨10.1371/journal.pone.0190014⟩
PLoS ONE, Vol 13, Iss 1, p e0190014 (2018)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0190014⟩
Popis: International audience; Background: Calcium silicate-based cements are biomaterials with calcium oxide and carbonate filler additives. Their properties are close to those of dentin, making them useful in restorative dentistry and endodontics. The aim of this study was to evaluate the in vitro biological effects of two such calcium silicate cements, Biodentine (BD) and Bioroot (BR), on dental stem cells in both direct and indirect contact models. The two models used aimed to mimic reparative dentin formation (direct contact) and reactionary dentin formation (indirect contact). An original aspect of this study is the use of an interposed thin agarose gel layer to assess the effects of diffusible components from the materials.Results: The two biomaterials were compared and did not modify dental pulp stem cell (DPSC) proliferation. BD and BR showed no significant cytotoxicity, although some cell death occurred in direct contact. No apoptosis or inflammation induction was detected. A striking increase of mineralization induction was observed in the presence of BD and BR, and this effect was greater in direct contact. Surprisingly, biomineralization occurred even in the absence of mineralization medium. This differentiation was accompanied by expression of odontoblast-associated genes. Exposure by indirect contact did not stimulate the induction to such a level.Conclusion: These two biomaterials both seem to be bioactive and biocompatible, preserving DPSC proliferation, migration and adhesion. The observed strong mineralization induction through direct contact highlights the potential of these biomaterials for clinical application in dentin-pulp complex regeneration.
Databáze: OpenAIRE
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