The ParD-mutant of Escherichia coli also carries Aammutation. The complete sequence of gyrA
| Autor: | E. J. Elliott, George P. C. Salmond, K. Hussain |
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| Rok vydání: | 1987 |
| Předmět: |
Transcription
Genetic Molecular Sequence Data Mutant Biology medicine.disease_cause Microbiology DNA gyrase Gene product Complete sequence Bacterial Proteins Sequence Homology Nucleic Acid Escherichia coli medicine Animals Amino Acid Sequence Molecular Biology Gene Genetics Base Sequence Nucleic acid sequence Chromosomes Bacterial biochemical phenomena metabolism and nutrition Molecular biology Genes Genes Bacterial Mutation Mutation (genetic algorithm) Bacillus subtilis |
| Zdroj: | Molecular Microbiology. 1:259-273 |
| ISSN: | 1365-2958 0950-382X |
| DOI: | 10.1111/j.1365-2958.1987.tb01932.x |
| Popis: | Summary The phenotype of a recently-described mutant (OVG), conditionally defective in chromosome partitioning and septal positioning, was originally thought to be due to a new gene (parD) mapping at 88.4 min. We have now shown that, in addition to the parD mutation, OV6 carries a gyrAam mutation and that this mutation is probably responsible for the gross phenotype of the mutant. We have cloned the gyrA gene, identified the GyrA protein, sequenced the gyrA gene and flanking genes, cloned and sequenced the gyrAam, mutation, and identified its truncated product, in addition, we have identified the transcriptional start point of the gyrA gene. The E. coli GyrA protein has extensive homologies with Gyrase proteins of other organisms and weak sequence homologies with some eukaryotic cytoskeletal proteins. |
| Databáze: | OpenAIRE |
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