Adaptation of the zymosan assay of properdin to animal sera
| Autor: | Andre J. Toussaint, William A. Hook, John F. Kent |
|---|---|
| Rok vydání: | 1957 |
| Předmět: |
Properdin
Acclimatization Zymosan chemical and pharmacologic phenomena Biology urologic and male genital diseases Molecular biology Adaptation Physiological female genital diseases and pregnancy complications General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound chemistry Polysaccharides Yeasts Chicken serum Animal species |
| Zdroj: | Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.). 96(3) |
| ISSN: | 0037-9727 |
| Popis: | SummaryThe reaction of zymosan with properdin of certain animal species was found to be inhibited in the presence of properdin-free human complement (RP), an essential reagent in the standard assay. The problem was resolved by 1) precipitating the properdin as euglobulin and reconstituting for the assay in properdin-free residues (R3 or endpiece) of human complement, or 2) deferring the addition of RP until properdin-zymosan complex had formed. The methods were applied successfully in the assay of guinea-pig, swine, and canine properdin, but were ineffective in demonstrating properdin in chicken serum. |
| Databáze: | OpenAIRE |
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