Construction of a System for the Strawberry Nursery Production towards Elimination of Latent Infection of Anthracnose Fungi by a Combination of PCR and Microtube Hybridization
| Autor: | Chikara Masuta, Saki Nagashima, Kazuyoshi Furuta, Tsuyoshi Inukai |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine microtube-hybridization biology Specific detection fungi Pcr cloning lcsh:Plant culture Note biology.organism_classification 01 natural sciences Glomerella cingulata DNA extraction Microbiology 03 medical and health sciences 030104 developmental biology Colletotrichum acutatum Colletotrichum lcsh:SB1-1110 strawberry Agronomy and Crop Science Gene 010606 plant biology & botany Fungal hyphae |
| Zdroj: | The Plant Pathology Journal, Vol 33, Iss 1, Pp 80-86 (2017) The Plant Pathology Journal |
| ISSN: | 1598-2254 |
| DOI: | 10.5423/ppj.nt.05.2016.0132 |
| Popis: | One of the major problems in strawberry production is difficulty in diagnosis of anthracnose caused by Colletotrichum acutatum or Glomerella cingulata in latent infection stage. We here developed a diagnostic tool for the latent infection consisting of initial culturing of fungi, DNA extraction, synthesis of PCR-amplified probes and microtube hybridization (MTH) using a macroarray. The initial culturing step is convenient to lure the fungi out of the plant tissues, and to extract PCR-inhibitor-free DNA directly from fungal hyphae. For specific detection of the fungi, PCR primers were designed to amplify the fungal MAT1-2 gene. The subsequent MTH step using the PCR products as probes can replace the laborious electrophoresis step providing us sequence information and high-throughput screening. Using this method, we have conducted a survey for a few thousands nursery plants every year for three consecutive years, and finally succeeded in eliminating latent infection in the third year of challenge. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|