Fungal and plant gene expression in the Tulasnella calospora-Serapias vomeracea symbiosis provides clues about nitrogen pathways in orchid mycorrhizas

Autor: Mariangela Girlanda, Igor V. Grigoriev, Francis Martin, Valeria Fochi, Vasanth R. Singan, Annegret Kohler, Samuele Voyron, Walter Chitarra, Raffaella Balestrini, Erika Lindquist, Silvia Perotto, Kerrie Barry
Přispěvatelé: Dept Life Sci & Syst Biol, University of Turin, Instituto per la Protezione Sostenibile delle Plante (IPSP), Interactions Arbres-Microorganismes (IAM), Université de Lorraine (UL)-Institut National de la Recherche Agronomique (INRA), DOE Joint Genome Inst, MIUR, 'Compagnia di San Paolo' (Torino, Italy), US Department of Energy (DOE) Joint Genome Institute DE AC02 05CH11231 978, Laboratory of Excellence Advanced Research on the Biology of Tree and Forest Ecosystems (ARBRE) ANR 11 LABX 0002 01, US DOE through the Oak Ridge National Laboratory Scientific Focus Area for Genomics Foundational Sciences (Plant Microbe Interfaces Project), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), Balestrini, Raffaella
Rok vydání: 2016
Předmět:
0106 biological sciences
0301 basic medicine
Physiology
[SDV]Life Sciences [q-bio]
Nitrogen assimilation
Tulasnella
Plant Science
01 natural sciences
transcriptomics
Orchid mycorrhiza
Gene Expression Regulation
Plant
Mycorrhizae
nitrogen (N)
Biomass
Mycorrhiza
Mycelium
Phylogeny
biology
Biological Sciences
Fungal
mycorhize
fungal genome
orchid mycorrhiza
Tulasnella
Serapias
nitrogen
génome fongique
orchidée
ammonium transporters
Nitrogen
Plant Biology & Botany
Genes
Fungal
mycorrhiza
orchid mycorrhiza
Saccharomyces cerevisiae
Genes
Plant
Serapias
Fungal Proteins
03 medical and health sciences
Symbiosis
absorption azotée
Botany
Genetics
transfert d'azote
Serapias vomeracea
Orchidaceae
Nutrition
Agricultural and Veterinary Sciences
organic carbon
Basidiomycota
fungi
Genetic Complementation Test
gene expression
Plant
biology.organism_classification
carbone organique
transport intracellulaire
030104 developmental biology
Gene Expression Regulation
Genes
Mutation
010606 plant biology & botany
Zdroj: Fochi, V; Chitarra, W; Kohler, A; Voyron, S; Singan, VR; Lindquist, EA; et al.(2017). Fungal and plant gene expression in the Tulasnella calospora–Serapias vomeracea symbiosis provides clues about nitrogen pathways in orchid mycorrhizas. New Phytologist, 213(1), 365-379. doi: 10.1111/nph.14279. Lawrence Berkeley National Laboratory: Retrieved from: http://www.escholarship.org/uc/item/6mn2c1s7
New Phytologist
New Phytologist, Wiley, 2017, 213 (1), pp.365-379. ⟨10.1111/nph.14279⟩
The New phytologist, vol 213, iss 1
New phytologist (Online) 213 (2017): 365–379. doi:10.1111/nph.14279
info:cnr-pdr/source/autori:Fochi V., Chitarra W., Kohler A., Voyron S., Singan V.R., Lindquist E.A., Barry K.W., Girlanda M., Grigoriev I.V., Martin F., Balestrini R., Perotto S./titolo:Fungal and plant gene expression in the Tulasnella calospora-Serapias vomeracea symbiosis provides clues about nitrogen pathways in orchid mycorrhizas/doi:10.1111%2Fnph.14279/rivista:New phytologist (Online)/anno:2017/pagina_da:365/pagina_a:379/intervallo_pagine:365–379/volume:213
New Phytologist 1 (213), 365-379. (2017)
ISSN: 1469-8137
0028-646X
Popis: © 2016 The Authors. New Phytologist © 2016 New Phytologist Trust Orchids are highly dependent on their mycorrhizal fungal partners for nutrient supply, especially during early developmental stages. In addition to organic carbon, nitrogen (N) is probably a major nutrient transferred to the plant because orchid tissues are highly N-enriched. We know almost nothing about the N form preferentially transferred to the plant or about the key molecular determinants required for N uptake and transfer. We identified, in the genome of the orchid mycorrhizal fungus Tulasnella calospora, two functional ammonium transporters and several amino acid transporters but found no evidence of a nitrate assimilation system, in agreement with the N preference of the free-living mycelium grown on different N sources. Differential expression in symbiosis of a repertoire of fungal and plant genes involved in the transport and metabolism of N compounds suggested that organic N may be the main form transferred to the orchid host and that ammonium is taken up by the intracellular fungus from the apoplatic symbiotic interface. This is the first study addressing the genetic determinants of N uptake and transport in orchid mycorrhizas, and provides a model for nutrient exchanges at the symbiotic interface, which may guide future experiments.
Databáze: OpenAIRE
Externí odkaz: