Decreased interleukin-2 production in murine acute pancreatitis: Potential for immunomodulation
| Autor: | Inna Saporoschetz, K. Collins, Miriam Nestor, Mary L. Rodrick, John A. Mannick, Paul Curley, M. V. Mendez |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Lipopolysaccharides
Interleukin 2 T-Lymphocytes medicine.medical_treatment Mice Inbred Strains Lymphocyte Activation Sepsis Mice chemistry.chemical_compound Immune system Adjuvants Immunologic Splenocyte Animals Medicine Hepatology Ethionine business.industry Gastroenterology medicine.disease Recombinant Proteins CD4 Lymphocyte Count Endotoxins Cytokine Pancreatitis chemistry Acute Disease Immunology Interleukin-2 Acute pancreatitis Female business medicine.drug |
| Zdroj: | Gastroenterology. 110:583-588 |
| ISSN: | 0016-5085 |
| DOI: | 10.1053/gast.1996.v110.pm8566607 |
| Popis: | The role of the cytokine interleukin 2 (IL-2) has long been recognized as central to normal immunologic function and defense against infection after burns and trauma, but little effort has been directed towards its role in acute pancreatitis (AP), which also has a high mortality related to sepsis. This study investigated the potential role of IL-2 in mice with diet-induced AP.AP was induced in mice by 10 days of feeding a choline-deficient, ethionine-supplemented diet. T-helper (CD4) cells were estimated, and T-cell mitogen-stimulated splenocyte proliferation and IL-2 production in vitro were measured on days 3, 7, and 10.Significant reduction in IL-2 production was found on day 3 (32%; P0.05) and day 10 (48%; P0.005). Administration of intraperitoneal lipopolysaccharide on day 10 was associated with reduced IL-2 production (P0.025) 4 hours later and 90% mortality in animals with AP. In vivo therapy with recombinant IL-2 improved in vitro IL-2 secretion (P0.05) and reduced lipopolysaccharide-induced mortality (P = 0.036).Murine diet-induced AP is associated with impaired immune function and increased susceptibility to sepsis and may be a valuable tool in the investigation of immunomodulation in AP. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|