High-performance liquid chromatographic study of the interactions between immobilized β-cyclodextrin polymers and hydrophobically end-capped polyethylene glycols
| Autor: | Christelle David, Marie Claude Millot, Bernard Sebille |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
chemistry.chemical_classification
Cyclodextrins Chromatography Cyclodextrin Polymers beta-Cyclodextrins technology industry and agriculture General Chemistry Polymer Reversed-phase chromatography Polyethylene glycol Polyethylene High-performance liquid chromatography Polyethylene Glycols Inclusion compound Hydrophobic effect chemistry.chemical_compound chemistry Organic chemistry Chromatography High Pressure Liquid |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 753:93-99 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/s0378-4347(00)00501-6 |
| Popis: | The formation of inclusion complexes between polyethylene glycols (PEGs) bearing hydrophobic ends (naphtyl and phenyladamantyl) and beta-cyclodextrin polymers (poly beta-CD) immobilized onto silica particles was studied by high-performance liquid chromatography (HPLC). It was shown that hydrophobic interactions were involved in the retention mechanism of these compounds, since retention volumes decreased when organic solvents were added to the mobile phase while it was the contrary in the presence of salts. Moreover, the association could be reversed by adding a competitor (hydroxypropyl beta-cyclodextrin) to the mobile phase. A theoretical model permitted the evaluation of affinity constants of 1:1 complexes formed between the modified PEGs and the immobilized poly beta-CD which depended on the type of hydrophobic groups grafted to the PEG. |
| Databáze: | OpenAIRE |
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