Combining microorganisms in inoculants is agronomically important but industrially challenging: case study of a composite inoculant containing Bradyrhizobium and Azospirillum for the soybean crop
| Autor: | Marcos Vinicios Conceição Garcia, Marco Antonio Nogueira, Mariangela Hungria |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Microorganism Biophysics Plant-growth promoting bacteria Industrial microbiology Microbiology 01 natural sciences Applied Microbiology and Biotechnology Bradyrhizobium Inoculation Bradyrhizobium diazoefficiens Microbial inoculant biology food and beverages 04 agricultural and veterinary sciences Biological nitrogen fixation Azospirillum brasilense biology.organism_classification QR1-502 Agronomy 040103 agronomy & agriculture Nitrogen fixation 0401 agriculture forestry and fisheries Original Article Beneficial organism TP248.13-248.65 Biotechnology 010606 plant biology & botany |
| Zdroj: | AMB Express AMB Express, Vol 11, Iss 1, Pp 1-13 (2021) |
| ISSN: | 2191-0855 |
| Popis: | The increasing global perception of the importance of microbial inoculants to promote productivity and sustainability in agriculture prompts the adoption of bio-inputs by the farmers. The utilization of selected elite strains of nitrogen-fixing and other plant-growth promoting microorganisms in single inoculants creates a promising market for composite inoculants. However, combining microorganisms with different physiological and nutritional needs requires biotechnological development. We report the development of a composite inoculant containing Bradyrhizobium diazoefficiens and Azospirillum brasilense for the soybean crop. Evaluation of use of carbon sources indicates differences between the microbial species, with Bradyrhizobium growing better with mannitol and glycerol, and Azospirillum with malic acid and maleic acid, allowing the design of a formulation for co-culture. Species also differ in their growth rates, and the best performance of both microorganisms occurred when Azospirillum was inoculated on the third day of growth of Bradyrhizobium. The composite inoculant developed was evaluated in five field trials performed in Brazil, including areas without and with naturalized populations of Bradyrhizobium. The composite inoculant resulted in symbiotic performance comparable to the application of the two microorganisms separately. In comparison to the single inoculation with Bradyrhizobium, co-inoculation resulted in average increases of 14.7% in grain yield and 16.4% in total N accumulated in the grains. The performance of the composite inoculant was similar or greater than that of the non-inoculated control receiving a high dose of N-fertilizer, indicating the importance of the development and validation of inoculants carrying multiple beneficial microorganisms. |
| Databáze: | OpenAIRE |
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