Identification of histone 3 variant 2 interacting factors
| Autor: | Monsef Benkirane, Daniel Latreille, Lisa Bluy, Rosemary Kiernan |
|---|---|
| Přispěvatelé: | Institut de génétique humaine (IGH), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
[SDV]Life Sciences [q-bio]
Cell Cycle Proteins Computational biology Gene Regulation Chromatin and Epigenetics Biology Cell Line S Phase Histones 03 medical and health sciences 0302 clinical medicine Histone H1 Histone methylation Histone H2A Genetics Humans Nucleosome Histone code Histone octamer ComputingMilieux_MISCELLANEOUS 030304 developmental biology Cell Nucleus 0303 health sciences Minichromosome Maintenance Proteins Chromatin 030220 oncology & carcinogenesis Histone methyltransferase Chromatin immunoprecipitation HeLa Cells Molecular Chaperones |
| Zdroj: | Nucleic Acids Research Nucleic Acids Research, Oxford University Press, 2014, 42 (6), pp.3542-3550. ⟨10.1093/nar/gkt1355⟩ |
| ISSN: | 0305-1048 1362-4962 |
| DOI: | 10.1093/nar/gkt1355⟩ |
| Popis: | The epigenome is defined as a type of information that can be transmitted independently of the DNA sequence, at the chromatin level, through post-translational modifications present on histone tails. Recent advances in the identification of histone 3 variants suggest a new model of information transmission through deposition of specific histone variants. To date, several non-centromeric histone 3 variants have been identified in mammals. Despite protein sequence similarity, specific deposition complexes have been characterized for both histone 3.1 (H3.1) and histone 3.3 (H3.3), whereas no deposition complex for histone 3.2 (H3.2) has been identified to date. Here, we identified human H3.2 partners by immunopurification of nuclear H3.2 complexes followed by mass spectrometry analysis. Further biochemical analyses highlighted two major complexes associated with H3.2, one containing chromatin associated factor-1 subunits and the other consisting of a subcomplex of mini chromosome maintenance helicases, together with Asf1. The purified complexes could associate with a DNA template in vitro. |
| Databáze: | OpenAIRE |
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