Identification of protective B-cell epitopes of Atroxlysin-I: A metalloproteinase from Bothrops atrox snake venom

Autor: Francisco Santos Schneider, F. Molina, Clara Guerra-Duarte, Carlos Chávez-Olórtegui, S. de Almeida Lima, G. Reis de Ávila, Claude Granier, Eladio F. Sanchez, K.L. Castro, Christophe Nguyen
Přispěvatelé: Sys2Diag-Modélisation et Ingénierie des Systèmes Complexes Biologiques pour le Diagnostic (Sys2Diag), Centre National de la Recherche Scientifique (CNRS)-Alcediag, Inserm, Instituto de Fisica Corpuscular (IFIC), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC)-Universitat de València (UV)
Rok vydání: 2016
Předmět:
0301 basic medicine
[SDV]Life Sciences [q-bio]
Molecular Sequence Data
Antivenom
Cross Reactions
medicine.disease_cause
Epitope
Microbiology
03 medical and health sciences
Western blot
Neutralization Tests
medicine
Animals
Bothrops
Amino Acid Sequence
ComputingMilieux_MISCELLANEOUS
Mice
Inbred BALB C
030102 biochemistry & molecular biology
General Veterinary
General Immunology and Microbiology
biology
medicine.diagnostic_test
Antivenins
Toxin
Public Health
Environmental and Occupational Health
Metalloendopeptidases
biology.organism_classification
Antibodies
Neutralizing
Molecular biology
Protein Structure
Tertiary
3. Good health
030104 developmental biology
Infectious Diseases
Epitope mapping
Snake venom
biology.protein
Epitopes
B-Lymphocyte
Molecular Medicine
Antibody
Peptides
Epitope Mapping
Snake Venoms
Zdroj: Vaccine
Vaccine, Elsevier, 2016, 34 (14), pp.1680-1687. ⟨10.1016/j.vaccine.2016.02.035⟩
ISSN: 0264-410X
DOI: 10.1016/j.vaccine.2016.02.035
Popis: Atroxlysin-I (Atr-I) is a hemorrhagic snake venom metalloproteinase (SVMP) from Bothrops atrox venom, the snake responsible for the majority of bites in the north region of South America. SVMPs like Atr-I produce toxic effects in victims including hemorrhage, inflammation, necrosis and blood coagulation deficiency. Mapping of B-cell epitopes in SVMPs might result in the identification of non-toxic molecules capable of inducing neutralizing antibodies and improving the anti-venom therapy. Here, using the SPOT-synthesis technique we identified two epitopes located in the N-ter region of Atr-I (AtrEp1-(22)YNGNSDKIRRRIHQM(36); and AtrEp2-(55)GVEIWSNKDLINVQ(68)). Based on the sequence of AtrEp1 and AtrEp2 a third peptide named Atr-I biepitope (AtrBiEp) was designed and synthesized ((23)NGNSDKIRRRIH(34)GG(55)GVEIWSNKDLINVQ(68)). AtrBiEp was used to immunize BALB/c mice. Anti-AtrBiEp serum cross-reacted against Atr-I in western blot and was able to fully neutralize the hemorrhagic activity of Atr-I. Our results provide a rational basis for the identification of neutralizing epitopes on Atr-I snake venom toxin and show that the use of synthetic peptides could improve the generation of immuno-therapeutics.
Databáze: OpenAIRE
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