Pseudomonas aeruginosa and Periodontal Pathogens in the Oral Cavity and Lungs of Cystic Fibrosis Patients: a Case-Control Study
Autor: | Sylvie Boisramé, G. Barbier, Michèle Virmaux, Geneviève Héry-Arnaud, K. Revert, S. Gouriou, Florence Le Gall, Gilles Rault, Rocio Rivas Caldas |
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Rok vydání: | 2015 |
Předmět: |
Adult
Male Microbiology (medical) Saliva Adolescent Cystic Fibrosis Dental Plaque Biology medicine.disease_cause Cystic fibrosis Microbiology Young Adult fluids and secretions medicine Humans Child ΔF508 Mouth Lung Pseudomonas aeruginosa Sputum Bacteriology Pathogenic bacteria medicine.disease Electrophoresis Gel Pulsed-Field Cross-Sectional Studies medicine.anatomical_structure Case-Control Studies Female Anaerobic bacteria medicine.symptom |
Zdroj: | Journal of Clinical Microbiology. 53:1898-1907 |
ISSN: | 1098-660X 0095-1137 |
Popis: | Cystic fibrosis (CF) is the most frequent lethal genetic disease in the Caucasian population. Lung destruction is the principal cause of death by chronic Pseudomonas aeruginosa colonization. There is a high prevalence of oropharyngeal anaerobic bacteria in sputum of CF patients. This study was carried out due to the lack of results comparing subgingival periodontal pathogenic bacteria between the oral cavity and lungs in patients with CF in relation with P. aeruginosa presence. Our first goal was to detect P. aeruginosa in oral and sputum samples by culture and molecular methods and to determine clonality of isolates. In addition, subgingival periodontal anaerobic bacteria were searched for in sputum. A cross-sectional pilot case-control study was conducted in the CF Reference Center in Roscoff, France. Ten CF patients with a ΔF508 homozygous mutation (5 chronically colonized [CC] and 5 not colonized [NC]) were enrolled. P. aeruginosa was detected in saliva, sputum, and subgingival plaque samples by real-time quantitative PCR (qPCR). Subsequently, periodontal bacteria were also detected and quantified in subgingival plaque and sputum samples by qPCR. In CC patients, P. aeruginosa was recovered in saliva and subgingival plaque samples. Sixteen P. aeruginosa strains were isolated in saliva and sputum from this group and compared by pulsed-field gel electrophoresis (PFGE). Subgingival periodontal anaerobic bacteria were found in sputum samples. A lower diversity of these species was recovered in the CC patients than in the NC patients. The presence of the same P. aeruginosa clonal types in saliva and sputum samples underlines that the oral cavity is a possible reservoir for lung infection. |
Databáze: | OpenAIRE |
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