Vanadium complex: an appropriate candidate for killing hepatocellular carcinoma cancerous cells
| Autor: | Hamid Bakhshi Aliabad, Hadis Ahmadirad, Mohammad Reza Hajizadeh, Mehdi Mahmoodi, Maryam Mohamadi, Soudeh Khanamani Falahati-Pour |
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| Rok vydání: | 2018 |
| Předmět: |
Carcinoma
Hepatocellular Necrosis Hepatocellular carcinoma Cytotoxicity Antineoplastic Agents 010402 general chemistry 01 natural sciences Article General Biochemistry Genetics and Molecular Biology Biomaterials Mice Structure-Activity Relationship 03 medical and health sciences 0302 clinical medicine Annexin Organometallic Compounds Pi medicine Animals Humans MTT assay HepG2 cells IC50 Cell Proliferation Cell Death Dose-Response Relationship Drug Chemistry Liver Neoplasms Metals and Alloys Vanadium Hep G2 Cells Vanadium complex medicine.disease Molecular biology L929 cells 0104 chemical sciences Apoptosis 030220 oncology & carcinogenesis Drug Screening Assays Antitumor medicine.symptom General Agricultural and Biological Sciences |
| Zdroj: | Biometals |
| ISSN: | 1572-8773 0966-0844 |
| DOI: | 10.1007/s10534-018-0139-x |
| Popis: | Hepatocellular carcinoma (HCC) is a prevalent human malignancy which its drug resistance is increasing world-wide. This project was designed to assess the anti-cancer effects of 4-bromo-2-(((5-chloro-2-hydroxyphenyl) imino) methyl) phenol ([IV(L)] complex) on the HepG2 cell line and also L929 cells, as normal cells. HepG2 and L929 cells were cultured in RPMI culture medium and the survival rates of the cells were determined after 24 and 48 h using MTT assay to find IC50 concentration of vanadium m, [IV(L)] complex. The early apoptosis and necrosis/late apoptosis were determined by means of annexin V/PI apoptosis detection kit. The results revealed that vanadium m, [IV(L)] complex induce early apoptosis higher in HepG2 cell line than L929 cells. The rates of necrosis/late apoptosis were also induced in HepG2 cells more than L929 cells. Based on the results, vanadium m, [IV(L)] complex might be considered as a safe new drug for treatment of HCC with low side effects on control liver cells. |
| Databáze: | OpenAIRE |
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