Ccr5 Regulates Inflammatory Gene Expression in Response to Encephalomyocarditis Virus Infection
| Autor: | Jason M. Moran, Benjamin S. Christmann, R. Mark L. Buller, Jennifer A. McGraw, John A. Corbett |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Interferon-Induced Helicase
IFIH1 Receptors CCR5 viruses Gene Expression Nitric Oxide Synthase Type II Biology Virus Pathology and Forensic Medicine DEAD-box RNA Helicases Chemokine receptor Mice Gene expression Animals Encephalomyocarditis virus Receptor Gene knockout Cells Cultured Macrophages NF-kappa B Regular Article NFKB1 Virology Antibodies Neutralizing Toll-Like Receptor 3 Mice Inbred C57BL Viral replication Cyclooxygenase 2 Interferon Type I Signal transduction Mitogen-Activated Protein Kinases Signal Transduction |
| Popis: | Encephalomyocarditis virus (EMCV) is capable of stimulating inflammatory gene expression by macrophages as a result of interactions between EMCV capsid proteins and cell surface receptors. In this study, biochemical and genetic approaches identified a role for Ccr5, a chemokine receptor, in transducing the signals of EMCV infection that result in the expression of inflammatory genes in macrophages. Antibody neutralization and gene knockout strategies were used to show that the presence of Ccr5 is required for EMCV-stimulated mitogen-activated protein (MAP) kinase and nuclear factor-kappa B (NF-κB) activation, and the subsequent expression of the inflammatory gene–inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2). Ccr5 appears to participate in the early control of virus replication: EMCV mRNA accumulates to sevenfold higher levels in Ccr5-deficient mice when compared to wild-type controls. These findings support a regulatory role for Ccr5 in the antiviral response to EMCV in which this chemokine receptor participates in regulation of inflammatory gene expression in response to virus infection. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|