Characterization of Flavonol Synthase and Leucoanthocyanidin Dioxygenase Genes in Arabidopsis (Further Evidence for Differential Regulation of 'Early' and 'Late' Genes)
| Autor: | Matthew K. Pelletier, Brenda W. Shirley, Julie R. Murrell |
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| Rok vydání: | 1997 |
| Předmět: |
musculoskeletal diseases
Light Physiology Molecular Sequence Data Mutant Arabidopsis Plant Science Biology Genes Plant Petunia Gene Expression Regulation Enzymologic Homology (biology) Gene Expression Regulation Plant Gene expression Genetics Flavonol synthase Amino Acid Sequence Cloning Molecular Gene Plant Proteins Sequence Tagged Sites Solanum tuberosum Expressed sequence tag Sequence Homology Amino Acid Gene Expression Regulation Developmental food and beverages Darkness Plants biology.organism_classification Multigene Family Oxygenases biology.protein Oxidoreductases Information Systems Research Article |
| Zdroj: | ResearcherID |
| ISSN: | 1532-2548 0032-0889 |
| DOI: | 10.1104/pp.113.4.1437 |
| Popis: | As part of an ongoing investigation into the organization and regulation of the flavonoid biosynthetic pathway, two Arabidopsis thaliana expressed sequence tag (EST) clones (153O10T7 and YAY780) with high homology to leucoanthocyanidin dioxygenase (LDOX) or flavonol synthase (FLS) were identified. EST YAY780 was sequenced and found to encode a protein 49 to 78% identical to all LDOX sequences in the database. EST 153O10T7 was used to isolate a genomic clone encoding a protein with 59 to 61% sequence identity to petunia (Petunia hybrida) and potato (Solanum tuberosum) FLS. DNA blot analysis was used to screen the Arabidopsis genome for sequences related to FLS and LDOX and to determine the positions of the two clones on the RI map. The expression patterns of FLS and LDOX in etiolated seedlings moved to white light and in two putative regulatory mutants (ttg and tt8) were determined by RNA blot analysis. These studies indicate that FLS is an “early” flavonoid gene in Arabidopsis seedlings, whereas LDOX is a “late” gene. Furthermore, FLS is the first flavonoid enzyme identified in Arabidopsis that may be encoded by a gene family. |
| Databáze: | OpenAIRE |
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