FANCD2, FANCJ and BRCA2 cooperate to promote replication fork recovery independently of the Fanconi Anemia core complex
| Autor: | Stephanie L. Kelich, Indrajit Chaudhury, Maya Raghunandan, Alexandra Sobeck, Helmut Hanenberg |
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| Rok vydání: | 2015 |
| Předmět: |
DNA Replication
congenital hereditary and neonatal diseases and abnormalities DNA repair Biology Origin of replication Models Biological Cell Line Aphidicolin Fanconi anemia hemic and lymphatic diseases FANCD2 medicine Humans Protein Isoforms Monoubiquitination Molecular Biology BRCA2 Protein Genetics Fanconi Anemia Complementation Group D2 Protein Ubiquitination DNA replication nutritional and metabolic diseases Helicase Cell Biology medicine.disease Chromatin Fanconi Anemia Complementation Group Proteins Basic-Leucine Zipper Transcription Factors Multiprotein Complexes biology.protein Mutant Proteins Protein Binding Signal Transduction Reports Developmental Biology |
| Zdroj: | Cell Cycle. 14:342-353 |
| ISSN: | 1551-4005 1538-4101 |
| DOI: | 10.4161/15384101.2014.987614 |
| Popis: | Fanconi Anemia (FA) is an inherited multi-gene cancer predisposition syndrome that is characterized on the cellular level by a hypersensitivity to DNA interstrand crosslinks (ICLs). To repair these lesions, the FA pathway proteins are thought to act in a linear hierarchy: Following ICL detection, an upstream FA core complex monoubiquitinates the central FA pathway members FANCD2 and FANCI, followed by their recruitment to chromatin. Chromatin-bound monoubiquitinated FANCD2 and FANCI subsequently coordinate DNA repair factors including the downstream FA pathway members FANCJ and FANCD1/BRCA2 to repair the DNA ICL. Importantly, we recently showed that FANCD2 has additional independent roles: it binds chromatin and acts in concert with the BLM helicase complex to promote the restart of aphidicolin (APH)-stalled replication forks, while suppressing the firing of new replication origins. Here, we show that FANCD2 fulfills these roles independently of the FA core complex-mediated monoubiquitination step. Following APH treatment, nonubiquitinated FANCD2 accumulates on chromatin, recruits the BLM complex, and promotes robust replication fork recovery regardless of the absence or presence of a functional FA core complex. In contrast, the downstream FA pathway members FANCJ and BRCA2 share FANCD2's role in replication fork restart and the suppression of new origin firing. Our results support a non-linear FA pathway model at stalled replication forks, where the nonubiquitinated FANCD2 isoform – in concert with FANCJ and BRCA2 – fulfills a specific function in promoting efficient replication fork recovery independently of the FA core complex. |
| Databáze: | OpenAIRE |
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