Induction of Bach1 and ARA70 gene expression at an early stage of adipocyte differentiation of mouse 3T3-L1 cells
| Autor: | Nishizuka, Makoto, Tsuchiya, Tomoko, Nishihara, Tsutomu, Imagawa, Masayoshi |
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| Rok vydání: | 2002 |
| Předmět: |
Oncogene Proteins
Mice Inbred C3H DNA Complementary Time Factors Nuclear Receptor Coactivators Nucleic Acid Hybridization Cell Differentiation 3T3 Cells Cell Biology Blotting Northern Biochemistry Mice Basic-Leucine Zipper Transcription Factors Adipocytes Trans-Activators Animals RNA RNA Messenger Cloning Molecular Molecular Biology Cell Division Research Article Signal Transduction Transcription Factors |
| Zdroj: | Biochemical Journal. 361:629-633 |
| ISSN: | 1470-8728 0264-6021 |
| DOI: | 10.1042/bj3610629 |
| Popis: | Using a subtraction method, we have isolated genes that are induced early in the differentiation of mouse 3T3-L1 preadipocyte cells into adipocytes. These include the genes encoding transcription factors and signalling proteins, as well as unknown genes. Bach1, a transcription factor, and ARA70, a cofactor, were rapidly induced during differentiation. The induction of these two genes was observed only in growth-arrested 3T3-L1 cells, and not in proliferating cells. In NIH-3T3 cells, no induction was observed under either set of conditions. These results strongly indicate that Bach1 and ARA70 have valuable roles at the onset of adipocyte differentiation. |
| Databáze: | OpenAIRE |
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