Phenolic compounds from the bark of Oroxylum indicum activate the Ngn2 promoter
| Autor: | Midori A. Arai, Rolly G. Fuentes, Samir Kumar Sadhu, Firoj Ahmed, Masami Ishibashi |
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| Rok vydání: | 2015 |
| Předmět: |
biology
Molecular Structure Plant Extracts Nerve Tissue Proteins biology.organism_classification Oroxylum indicum Molecular biology Baicalein chemistry.chemical_compound chemistry Phenols Apigenin Basic Helix-Loop-Helix Transcription Factors Molecular Medicine Oroxylin A Hispidulin Chrysin Transcription factor Baicalin Transcription Factors |
| Zdroj: | Journal of natural medicines. 69(4) |
| ISSN: | 1861-0293 |
| Popis: | A reporter gene assay that detects neurogenin 2 (Ngn2) promoter activity was utilized to identify compounds that induce neuronal differentiation. Ngn2 is a basic helix-loop-helix transcription factor that activates transcription of pro-neural genes. Using this assay system and an activity-guided approach, seven phenolic compounds were isolated from the methanol extract of Oroxylum indicum: 1 oroxylin A, 2 chrysin, 3 hispidulin, 4 baicalein, 5 apigenin, 6 baicalin, and 7 isoverbascoside. Compounds 1 and 2 induced an estimated 2.7-fold increase in Ngn2 promoter activity, whereas 3 increased the activity by 2.5-fold. Furthermore, 1 and 2 enhanced neuronal differentiation of C17.2 cells, which are multipotent stem cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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