Distribution of neuropeptide W in the rat brain
| Autor: | Kei Endo, Fumiko Takenoya, Michiko Yagi, Kanako Shiba, Yukari Date, Seiji Shioda, Haruaki Kageyama, Masamitsu Nakazato, Naoko Nonaka |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Lateral hypothalamus Hypothalamus Gene Expression Supraoptic nucleus Rats Sprague-Dawley Cellular and Molecular Neuroscience Nerve Fibers Endocrinology Arcuate nucleus Internal medicine medicine Animals Lateral parabrachial nucleus RNA Messenger Rats Wistar Neurons Reverse Transcriptase Polymerase Chain Reaction Endocrine and Autonomic Systems Chemistry Neuropeptides General Medicine Neuropeptide W Amygdala Immunohistochemistry Rats Preoptic area Microscopy Electron Stria terminalis nervous system Neurology Nerve Net Brain Stem |
| Zdroj: | Neuropeptides. 44:99-106 |
| ISSN: | 0143-4179 |
| DOI: | 10.1016/j.npep.2009.10.007 |
| Popis: | Neuropeptide W (NPW), which was recently isolated from the porcine hypothalamus, has been identified as the endogenous ligand of the orphan G protein-coupled receptors GPR7 (NPBWR1) and GPR8 (NPBWR2). Infusion of NPW increases food intake in the light phase, whereas in the dark phase, it has the opposite effect. In this study, we used RT-PCR analysis to examine the gene expression of NPW mRNA in the rat brain, and performed a detailed analysis of the distribution of NPW-positive neurons by use of immunohistochemistry at both the light and electron microscopic levels. NPW mRNA expression was demonstrated in the hypothalamic paraventricular nucleus (PVN), arcuate nucleus (ARC), ventromedial nucleus (VMH) and lateral hypothalamus (LH). At the light microscopic level, NPW-like immunoreactive (NPW-LI) cell bodies were found in the preoptic area (POA), PVN, ARC, VMH, LH, PMD (dorsal premammillary nucleus), periaqueductal gray (PAG), lateral parabrachial nucleus (LPB), and prepositus nucleus (Pr). NPW-LI axon terminals were shown in the POA, bed nucleus of the stria terminalis (BST), amygdala, PVN, ARC, VMH, LH, and PAG, LPB. In addition, at the electron microscopic level, NPW-LI cell bodies and dendritic processes were often seen to receive inputs from other unknown neurons in the ARC, PVN, VMH and amygdala. Our observations indicate that NPW-LI neurons widely distributed in the rat brain region. These finding suggest that NPW may have important roles in feeding behavior, energy homeostasis, emotional response and regulation of saliva secretion. |
| Databáze: | OpenAIRE |
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