| Autor: |
Yanhui Zhang, Xiaofei Liang, Mengxin Zhao, Tuo Qi, Hualong Guo, Jing Zhao, Jie Zhao, Gangming Zhan, Zhensheng Kang, Li Zheng |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Plant communications. |
| ISSN: |
2590-3462 |
| Popis: |
Here, we describe a novel narnavirus termed Puccinia striiformis virus 5 (PsV5) from the devastating wheat stripe rust fungus Puccinia striiformis f. sp. tritici (Pst). The genome of PsV5 contains two predicted open reading frames (ORFs) that are largely overlapped on reverse strands, a RNA-dependent RNA polymerase (RdRp) and a reverse-frame ORF (rORF) with unknown function. Protein translations of both ORFs were demonstrated by immune technology. Transgenic wheat lines overexpressing PsV5 (RdRp-rORF), RdRp ORF or rORF were more susceptible to Pst infection whereas PsV5-RNAi lines were more resistant. Overexpression of PsV5 (RdRp-rORF), RdRp ORF or rORF in Fusarium graminearum also boosted fungal virulence. Together, we report a novel ambigrammatic mycovirus that promotes the virulence of its fungal host. The results are a significant addition to the knowledge of the virosphere diversity and offers insights for sustainable wheat rust disease control. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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