Isolation and characterization of a retrovirus from the fish genus Xiphophorus
| Autor: | Kerstin Petry, Markus Schmidt, Fritz Anders, Harald Petry, Gerhard Hunsmann, Wolfgang Loke |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Base Sequence
biology Molecular Sequence Data Nucleic Acid Hybridization RNA-Directed DNA Polymerase Xiphophorus biology.organism_classification Genes gag Feline leukemia virus Molecular biology Reverse transcriptase Virus Cyprinodontiformes Microscopy Electron Retroviridae Retrovirus Oligodeoxyribonucleotides Sequence Homology Nucleic Acid Virology Nucleic acid Animals Northern blot Southern blot |
| Zdroj: | Virology. 188:785-792 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/0042-6822(92)90533-u |
| Popis: | A cell line (BsT) established from neoplastic embryonal tissues of the platyfish ( Xiphophorus maculatus ) released spontaneously retrovirus-like particles. The particles have a buoyant density of 1.16 g/ml, a mean diameter of 100 nm and the morphology of immature retroviruses. The particle-associated proteins p70, p65, and p28 react with an antiserum directed against the major internal feline leukemia virus structural protein p27. The particles are associated with a reverse transcriptase. The purified enzyme has a molecular weight of about 70 kDa and prefers the template primers poly(rA):oligo(dT), poly(dC):oligo(dG), and poly(rC):oligo(dG) in the presence of Mn 2+ . The enzyme activity is inhibited by antibodies directed against the reverse transcriptase of feline leukemia virus and simian sarcoma virus. The particles contain a ribonucleic acid of about 70 S. In an endogenous reverse transcriptase reaction nucleic acids in the range of 0.2 to 0.4 kb were synthesized. In Northern blots with these nucleic acids as probe, three transcripts of about 8.5, 4.2, and 1.5 kb were detected in BsT cells. Southern blot analysis with the same probe demonstrates related sequences in the DNA of BsT cells and the platyfish and swordtail ( Xiphophorus hellerl ). Hybridization experiments with the LTR-gag region of the feline leukemia virus show homologous sequences in the Xiphophorus genome . |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|