Experimental application of the median-effect principle for in vitro quantification of the combined inhibitory activities of clavulanic acid and imipenem against IRT-4 β-lactamase
| Autor: | Vincent Foulongne, J. Jourdan, Roger Labia, Albert Sotto, D. Sirot |
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| Rok vydání: | 2002 |
| Předmět: |
Microbiology (medical)
Imipenem medicine.drug_class Cephalosporin Microbial Sensitivity Tests beta-Lactamases Clavulanic Acids Inhibitory Concentration 50 Clavulanic acid Escherichia coli medicine Pharmacology (medical) Enzyme Inhibitors Antibacterial agent chemistry.chemical_classification biology Chemistry Drug Resistance Microbial Drug Synergism General Medicine Models Theoretical biology.organism_classification Enterobacteriaceae In vitro Anti-Bacterial Agents Infectious Diseases Enzyme Biochemistry Enzyme inhibitor biology.protein beta-Lactamase Inhibitors medicine.drug |
| Zdroj: | International Journal of Antimicrobial Agents. 19:75-78 |
| ISSN: | 0924-8579 |
| DOI: | 10.1016/s0924-8579(01)00465-4 |
| Popis: | Inhibitor-resistant TEM β-lactamases (IRT) have been identified in Enterobacteriaceae which, however, remained susceptible to cephalosporins. We evaluated the combined inhibitory activity of clavulanic acid and imipenem at ratios of 1:1 and 1:3 against IRT-4, using the median effect principle of Chou and Talalay. The combination of the two drugs, which produced a nearly additive effect, meant their concentrations could be lowered 1.3–4.9-fold, while maintaining a 50% inhibitory effect against the IRT-4 in comparison with each drug alone. From a therapeutic point of view, such a combination is not efficient but this method of Chou and Talalay, used for the first time to assay combined inhibitory activity of β-lactamase inhibitors, could be used to evaluate new molecules and/or strategies to inactivate β-lactamase. |
| Databáze: | OpenAIRE |
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