Interleukin-6 changes deformability of neutrophils and induces their sequestration in the lung
| Autor: | Tatsushi Suwa, Maria E. Klut, Jennifer Hards, Stephan F. van Eeden, James C. Hogg |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Pulmonary and Respiratory Medicine
Pathology medicine.medical_specialty Neutrophils Neutrophile medicine.medical_treatment Critical Care and Intensive Care Medicine Sensitivity and Specificity Leukocyte Count Bone Marrow Reference Values Culture Techniques medicine Animals Bronchopulmonary Sequestration Interleukin 6 Lung Probability Analysis of Variance biology Dose-Response Relationship Drug business.industry Interleukin-6 Molecular biology Neutrophilia In vitro Actins Elasticity medicine.anatomical_structure Cytokine biology.protein Liberation Female Bone marrow Rabbits medicine.symptom Cell activation business |
| Zdroj: | American journal of respiratory and critical care medicine. 163(4) |
| ISSN: | 1073-449X |
| Popis: | Interleukin-6 (IL-6) is an important mediator of both the hepatic and the bone marrow components of the acute-phase response. Previous studies from our laboratory have shown that cells released into the circulation from the marrow preferentially sequester in the lung. The present study was designed to examine the mechanism of this sequestration using a single dose of recombinant human IL-6 to stimulate the marrow in rabbits. Marrow release was monitored by labeling polymorphonuclear leukocyte (PMN) precursors in the marrow with the thymidine analogue, 5'-bromo-2-deoxyuridine (BrdU), 24 h before IL-6 treatment. This treatment caused a neutrophilia that was associated with the increase of circulating BrdU- labeled PMN (PMN(BrdU)) and morphometric studies confirmed that PMN(BrdU) released from the marrow preferentially sequestered in the lung microvessels compared to unlabeled PMN. IL-6 treatment increases PMN F-actin content (p < 0.05) that was not due to cell activation by IL-6. In vitro studies show that IL-6 treatment decreased the deformability of circulating PMN (p < 0.05). These studies confirm that IL-6 treatment causes an accelerated release of PMN from the bone marrow and shows that these newly released PMN have high levels of F-actin, are less deformable, and preferentially sequester in lung microvessels. |
| Databáze: | OpenAIRE |
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