17beta-Estradiol metabolites affect some regulators of the MCF-7 cell cycle
| Autor: | J.C. Seegers, Christina J.S. Grobler, Theorickus C. Viljoen, Maryna de Kock, Mona-Liza Lottering |
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| Rok vydání: | 1996 |
| Předmět: |
Cancer Research
Cyclin-dependent kinase 1 biology Estradiol Cell growth Cell Cell cycle Proliferating cell nuclear antigen Cell biology S Phase medicine.anatomical_structure Oncology Biochemistry Mitogen-activated protein kinase Proliferating Cell Nuclear Antigen CDC2 Protein Kinase biology.protein medicine Tumor Cells Cultured Humans Protein phosphorylation Phosphorylation Protein kinase A |
| Zdroj: | Cancer letters. 110(1-2) |
| ISSN: | 0304-3835 |
| Popis: | The activity of p34(cdc2) plays a key role in the regulation of the eukaryotic cell cycle. Another cell cycle associated molecule is PCNA. We investigated the effects of 2-hydroxy-17beta-estradiol, a cell proliferator, and 2-methoxy-17beta-estradiol, a potent inhibitor of cell growth, on the levels and activity of p34(cdc2) and on the levels of PCNA, as well as on protein phosphorylation in MCF-7 cells. 2-Hydroxyestradiol increased p34(cdc2) activity at G1/S and elevated PCNA levels during S-phase. 2-Methoxyestradiol caused unscheduled activation of p34(cdc2) in S-phase and decreased levels of p34(cdc2) and PCNA during G2/M. We conclude that 2-hydroxy- and 2-methoxyestradiol have definite, though different regulatory functions during the cell cycle. |
| Databáze: | OpenAIRE |
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