Characterisation of class 3 integrons with oxacillinase gene cassettes in hospital sewage and sludge samples from France and Luxembourg
| Autor: | Pierrette Landrie Simo Tchuinte, Silvia Venditti, Olivier Barraud, Thibault Stalder, Christophe Dagot, Marie-Cécile Ploy, Antoinette Ngandjio |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Luxembourg 030106 microbiology Sewage Biology Real-Time Polymerase Chain Reaction Integron beta-Lactamases Aeromonas allosaccharophila Integrons Microbiology 03 medical and health sciences Antibiotic resistance Plasmid Drug Resistance Bacterial Humans Pharmacology (medical) Molecular Biology Bacteriological Techniques Acinetobacter business.industry High-Throughput Nucleotide Sequencing General Medicine biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Hospitals Citrobacter freundii 030104 developmental biology Infectious Diseases Gene cassette biology.protein bacteria Aeromonas France business Bacteria |
| Zdroj: | International Journal of Antimicrobial Agents. 48:431-434 |
| ISSN: | 0924-8579 |
| DOI: | 10.1016/j.ijantimicag.2016.06.018 |
| Popis: | In this study, antibiotic resistance class 3 integrons in Gram-negative bacteria isolated from hospital sewage and sludge and their genetic contents were characterised. Two samples of hospital effluent from France and Luxembourg and one sample of sludge from a wastewater treatment plant in France were collected in 2010 and 2011. Bacteria were cultured on selective agar plates and integrons were detected in colonies by quantitative PCR. Integron gene cassette arrays and their genetic environments were analysed by next-generation sequencing. Three class 3 integron-positive isolates were detected, including Acinetobacter johnsonii LIM75 (French hospital effluent), Aeromonas allosaccharophila LIM82 (sludge) and Citrobacter freundii LIM86 (Luxembourg hospital effluent). The gene cassettes were all implicated in antibiotic (aminoglycoside and β-lactam) or antiseptic resistance. An oxacillinase gene cassette (blaOXA-10, blaOXA-368 or blaOXA-2) was found in each integron. All of the class 3 integrons were located on small mobilisable plasmids. This study highlights the role of class 3 integrons in the dissemination of clinically relevant antibiotic resistance genes, notably oxacillinase genes, in hospital effluent. |
| Databáze: | OpenAIRE |
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