Better Preservation of the Peritoneum in Rats Exposed to Amino Acid-Based Peritoneal Dialysis Fluid
| Autor: | Liesbeth H P Hekking, Eelco D. Keuning, Mohammad Zareie, Anton A. van Lambalgen, Jacob van den Born, Inge L. Schadee-Eestermans, Piet M. ter Wee, Robert H.J. Beelen, Bart Degreve, Geert-Jan Tangelder, Dirk Faict |
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| Přispěvatelé: | Groningen Kidney Center (GKC), Groningen Institute for Organ Transplantation (GIOT) |
| Rok vydání: | 2005 |
| Předmět: |
Male
medicine.medical_specialty Time Factors Biocompatibility medicine.medical_treatment 030232 urology & nephrology Epithelium/drug effects Peritoneum/blood supply Peritoneal dialysis 03 medical and health sciences Amino Acids/chemistry 0302 clinical medicine Peritoneum Fibrosis Internal medicine medicine Animals Dialysis Solutions/chemistry 030212 general & internal medicine Rats Wistar chemistry.chemical_classification Neovascularization Pathologic/chemically induced business.industry Peritoneal fluid fungi General Medicine medicine.disease Rats Surgery Amino acid Microcirculation/drug effects body regions Mesothelium Endocrinology medicine.anatomical_structure nervous system chemistry Nephrology business Peritoneal Dialysis Kidney disease |
| Zdroj: | Peritoneal dialysis international, 25(1), 58-67. MULTIMED INC |
| ISSN: | 1718-4304 0896-8608 |
| DOI: | 10.1177/089686080502500112 |
| Popis: | BackgroundGlucose-containing peritoneal dialysis fluids (PDF) show impaired biocompatibility, which is related partly to their high glucose content, presence of glucose degradation products, low pH, and lactate buffer, or a combination of these factors. In a rat chronic peritoneal exposure model, we compared effects of an amino acid-based PDF (AA-PDF) with a glucose-containing PDF on the peritoneal microcirculation and morphology.MethodTwo groups of rats received 10 mL of either fluid daily for 5 weeks via peritoneal catheters connected to implanted subcutaneous mini vascular access ports. Leukocyte–endothelium interactions in the mesenteric venules were investigated by intravital microscopy. Quantification of angiogenesis and fibrosis and inspection of the mesothelial cell layer were performed by light and electron microscopy.ResultsDaily exposure to glucose-containing PDF resulted in a significant increase in the number of rolling leukocytes in mesenteric venules, whereas instillation of AA-PDF did not change the level of leukocyte rolling. Glucose-containing PDF evoked a significantly higher number of milky spots in the omentum, whereas this response was significantly reduced in animals exposed to the AA-PDF ( p < 0.02). Chronic instillation of glucose-containing PDF induced angiogenesis in various peritoneal tissues, accompanied by fibrosis in the mesentery and parietal peritoneum. Quantitative morphometric evaluation of omentum and mesentery showed a clear trend toward less angiogenesis after treatment with the AA-PDF compared to the glucose-containing PDF, which reached statistical significance in the parietal peritoneum ( p < 0.04). Instillation of AA-PDF resulted in approximately 50% reduction of fibrosis in the mesentery ( p < 0.04) and approximately 25% reduction in the parietal peritoneum ( p < 0.009) compared to glucose-containing PDF. Glucose-containing PDF damaged the mesothelial cell layer, whereas the mesothelium was intact after AA-PDF treatment, as evidenced by electron microscopy.ConclusionOur data in a rat chronic peritoneal exposure model clearly demonstrate reduced immune activation (evidenced by decreased number of rolling leukocytes and decreased induction of omental milky spots) and reduced neoangiogenesis, fibrosis, and mesothelial damage of the peritoneal membrane after treatment with AA-PDF compared to glucose-containing PDF. |
| Databáze: | OpenAIRE |
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