The Proliferation and Migration Effects of Huangqi on RSC96 Schwann Cells
| Autor: | Fu Yang Ko, Yueh Sheng Chen, Hwai Lee Wang, Chia-Hua Kuo, Chang Hai Tsai, Wen Kuei Fang, Chih Yang Huang, Li Mien Chen, Wei Wen Kuo, Fuu Jen Tsai |
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| Rok vydání: | 2009 |
| Předmět: |
MAPK/ERK pathway
Cell Survival Pyridines p38 mitogen-activated protein kinases Blotting Western Cyclin A Biology Models Biological p38 Mitogen-Activated Protein Kinases Cell Line Cyclin D1 Cell Movement Cyclin E Nitriles Butadienes Animals Enzyme Inhibitors Cell Proliferation Anthracenes Mitogen-Activated Protein Kinase 3 Dose-Response Relationship Drug Reverse Transcriptase Polymerase Chain Reaction Cell growth Imidazoles Astragalus Plant General Medicine Astragalus propinquus Cell cycle Urokinase-Type Plasminogen Activator Rats Cell biology Blot Matrix Metalloproteinase 9 Complementary and alternative medicine Fibroblast Growth Factor 2 Schwann Cells Signal transduction Wound healing Drugs Chinese Herbal |
| Zdroj: | The American Journal of Chinese Medicine. 37:945-959 |
| ISSN: | 1793-6853 0192-415X |
| DOI: | 10.1142/s0192415x09007363 |
| Popis: | This study evaluated the proliferation effects of huangqi on neuron regeneration. We investigated the molecular mechanisms, which include: (1) cyclin D1, A, E-cell cycle factors and MAPK signaling proliferation (2) FGF-2-UPA-MMPs migration signaling. After treatment with various Huanqi concentrations (1.25, 12.5, 125, 250 and 500 μg/ml,), we observed that Huanqi can increase Rsc 96 cell proliferation at 12.5 μg/ml ( p < 0.01) concentration determined by the MTT and wound healing tests. Examination by RT-PCR and Western blotting assay showed that Huangqi is able to stimulate the mRNA and protein expressions of cyclin D1, A, E, cell cycle controlling proteins and excite ERK and P38 MAPK signaling pathways to promote cell proliferation. Huangqi stimulates the FGF-2-UPA-MMP 9 migration pathway and enhances RSC 96 Schwann cells migration. Using MAPK chemical inhibitors, U0126, SB203580 and SP600125, the proliferative effects of Huangqi on RSC 96 cells were ERK and P38 signaling-dependent. Based on these results, applying an appropriate dose of Huangqi with biomedical materials would be a potential approach to enhancing neuron regeneration. |
| Databáze: | OpenAIRE |
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