Hypoxia promotes stem-like properties of laryngeal cancer cell lines by increasing the CD133+ stem cell fraction
| Autor: | Dawei Li, Huai-Dong Du, Chunping Wu, Lei Tao, Hongli Gong, Liang Zhou, Jie Tian |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Homeobox protein NANOG
Cancer Research Biology medicine.disease_cause SOX2 Antigens CD Cancer stem cell Cell Line Tumor Biomarkers Tumor medicine Humans AC133 Antigen Laryngeal Neoplasms Cell Proliferation Glycoproteins Cell growth Cell Cycle Cell cycle Cell Hypoxia Neoplasm Proteins Gene Expression Regulation Neoplastic Oncology Cancer cell Neoplastic Stem Cells Cancer research Stem cell Peptides Carcinogenesis |
| Zdroj: | International Journal of Oncology. 44:1652-1660 |
| ISSN: | 1791-2423 1019-6439 |
| DOI: | 10.3892/ijo.2014.2307 |
| Popis: | Evidence indicates that a hypoxic micro-environment plays an essential role in the regulation of cancer stem cells (CSCs). However, whether hypoxia is able to regulate the stem-like biological properties of laryngeal cancer cells remains unknown. In this study, we investigated the influence of hypoxia on the stemness of two laryngeal cancer cell lines, Hep-2 and AMC-HN-8. We cultured the two cell lines under hypoxia and normoxia and examined the influence of hypoxia on the expression of hypoxia-inducible factors (HIFs) and the cancer stem-like properties of these cells, including cell cycle distribution, expression of stem cell genes (OCT4, SOX2 and NANOG) and laryngeal CSC surface marker (CD133), proliferation, invasion, colony formation and sphere formation capacity. We determined that both of these cell lines, when maintained under hypoxic conditions, showed expanded cells in the G0/G1 phase, exhibited preferential expression of stem cell genes and CD133, and manifested upregulation of HIFs. When treated with hypoxia followed by normoxia exposure, the two cell lines exhibited enhanced capacities for proliferation, invasion, and sphere and colony formation compared with cells maintained consistently under normoxia. Our findings indicate that a hypoxic microenvironment may upgrade the stem-like biological properties of laryngeal cancer cell lines by the expansion of the CD133(+) stem cell fraction. |
| Databáze: | OpenAIRE |
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