| Autor: |
M. Busse, S. Ferstl, M. A. Kimm, L. Hehn, K. Steiger, S. Allner, M. Muller, E. Drecoll, T. Burkner, M. Dierolf, B. Gleich, W. Weichert, F. Pfeiffer |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
IEEE transactions on medical imaging. 41(12) |
| ISSN: |
1558-254X |
| Popis: |
Histopathology as a diagnostic mainstay for tissue evaluation is strictly a 2D technology. Combining and supplementing this technology with 3D imaging has been proposed as one future avenue towards refining comprehensive tissue analysis. To this end, we have developed a laboratory-based X-ray method allowing for the investigation of tissue samples in three dimensions with isotropic volume information. To assess the potential of our method for micro-morphology evaluation, we selected several kidney regions from three patients with cystic kidney disease, obstructive nephropathy and diabetic glomerulopathy. Tissue specimens were processed using our in-house-developed X-ray eosin stain and investigated with a commercial microCT and our in-house-built NanoCT. The microCT system provided overview scans with voxel sizes of [Formula: see text] and the NanoCT was employed for higher resolutions including voxel sizes from [Formula: see text] to 210 nm. We present a methodology allowing for a precise micro-morphologic investigation in three dimensions which is compatible with conventional histology. Advantages of our methodology are its versatility with respect to multi-scale investigations, being laboratory-based, allowing for non-destructive imaging and providing isotropic volume information. We believe, that after future developmental work this method might contribute to advanced multi-modal tissue diagnostics. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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