Studies on prevalence of biofilm associated genes and primary observation onsasX gene in clinical isolates of coagulase negative staphylococci (CoNS)

Autor:	Sheela Sugathan, E. K. Radhakrishnan, Kandammuriyil Radhakrishnan Soumya, Shiji Mathew, Jyothis Mathew
Rok vydání:	2016
Předmět:	Coagulase 0301 basic medicine Microbiology (medical) Staphylococcus aureus Genotype 030106 microbiology Gene Expression Microbial Sensitivity Tests Staphylococcal infections medicine.disease_cause Pathology and Forensic Medicine Microbiology 03 medical and health sciences Antibiotic resistance Bacterial Proteins Drug Resistance Multiple Bacterial medicine Humans Immunology and Allergy Prospective Studies Skin biology Biofilm General Medicine Staphylococcal Infections biochemical phenomena metabolism and nutrition biology.organism_classification medicine.disease Staphylococcus haemolyticus Anti-Bacterial Agents Bacterial Typing Techniques Multiple drug resistance 030104 developmental biology Genes Bacterial Biofilms Catheter-Related Infections Doxycycline
Zdroj:	APMIS. 124:319-326
ISSN:	0903-4641
Popis:	Coagulase negative staphylococci (CoNS) are nosocomial pathogens that cause indwelling medical device associated infections due to its biofilm forming potential and multiple antibiotic resistance. The current study focused on species identification, antibiotic resistance profile and molecular basis of biofilm formation and attachment of CoNS isolated from clinical samples. Along with this, molecular screening for mecA and newly identified surface colonization protein encoded by sasX gene was also conducted. S. epidermidis (n = 19, 47%) was identified as the most prevalent CoNS species and very interestingly two biofilm forming, mecA positive S. epidermidis isolates were found to carry all the biofilm associated genes screened in this study, which indicates its potential to form the strong biofilm. Another novel observation of the study is the detection of sasX gene in one biofilm positive S. epidermidis isolate. The study also identified one doxycycline resistant mecA positive, multidrug resistant S. haemolyticus isolate. In conclusion, the study signifies the existence of multiple biofilm related genes, multidrug resistance and the presence of sasX gene among clinical isolates of CoNS.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0b3778f43c9c7301d4032ac4ec634c14 https://doi.org/10.1111/apm.12510 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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