The Esterase PfeE, the Achilles’ Heel in the Battle for Iron between Pseudomonas aeruginosa and Escherichia coli
| Autor: | Philippe Hammann, Véronique Gasser, Laurianne Kuhn, Laurent Aussel, Isabelle J. Schalk, Thibaut Hubert |
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| Přispěvatelé: | Biotechnologie et signalisation cellulaire (BSC), Université de Strasbourg (UNISTRA)-Institut de recherche de l'Ecole de biotechnologie de Strasbourg (IREBS)-Centre National de la Recherche Scientifique (CNRS), Plateforme Protéomique Strasbourg - Esplanade (IBMC / CNRS FRC1589 / UNIV Strasbourg), Institut de biologie moléculaire et cellulaire (IBMC), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de chimie bactérienne (LCB), Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut de recherche de l'Ecole de biotechnologie de Strasbourg (IREBS), Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Moléculaire et Cellulaire [Strasbourg] (IBMC) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Siderophore TonB siderophore [SDV]Life Sciences [q-bio] 030106 microbiology education Sciences du Vivant [q-bio]/Biochimie Biologie Moléculaire medicine.disease_cause Esterase Catalysis outer membrane transporters Microbiology lcsh:Chemistry Inorganic Chemistry 03 medical and health sciences chemistry.chemical_compound Enterobactin medicine otorhinolaryngologic diseases Physical and Theoretical Chemistry lcsh:QH301-705.5 Molecular Biology Escherichia coli Spectroscopy biology Chemistry Pseudomonas aeruginosa co-cultures iron uptake Organic Chemistry General Medicine Periplasmic space biology.organism_classification Computer Science Applications 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 iron homeostasis enterobactin Bacterial outer membrane Bacteria |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 6 International Journal of Molecular Sciences, 2021, 22 (6), pp.2814. ⟨10.3390/ijms22062814⟩ International Journal of Molecular Sciences, MDPI, 2021, 22 (6), pp.2814. ⟨10.3390/ijms22062814⟩ International Journal of Molecular Sciences, Vol 22, Iss 2814, p 2814 (2021) |
| ISSN: | 1422-0067 1661-6596 |
| DOI: | 10.3390/ijms22062814 |
| Popis: | Bacteria access iron, a key nutrient, by producing siderophores or using siderophores produced by other microorganisms. The pathogen Pseudomonas aeruginosa produces two siderophores but is also able to pirate enterobactin (ENT), the siderophore produced by Escherichia coli. ENT-Fe complexes are imported across the outer membrane of P. aeruginosa by the two outer membrane transporters PfeA and PirA. Iron is released from ENT in the P. aeruginosa periplasm by hydrolysis of ENT by the esterase PfeE. We show here that pfeE gene deletion renders P. aeruginosa unable to grow in the presence of ENT because it is unable to access iron via this siderophore. Two-species co-cultures under iron-restricted conditions show that P. aeruginosa strongly represses the growth of E. coli as long it is able to produce its own siderophores. Both strains are present in similar proportions in the culture as long as the siderophore-deficient P. aeruginosa strain is able to use ENT produced by E. coli to access iron. If pfeE is deleted, E. coli has the upper hand in the culture and P. aeruginosa growth is repressed. Overall, these data show that PfeE is the Achilles’ heel of P. aeruginosa in communities with bacteria producing ENT. |
| Databáze: | OpenAIRE |
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