Differentiation behavior of iPS cells cultured on PLGA with osteoinduction medium
| Autor: | Reiko Tokita, Kei Nakajima, Kenichi Matsuzaka, Kenji Inoue, Takashi Inoue, Tungalag Ser-Od, Akram Al-Wahabi |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Materials science Induced Pluripotent Stem Cells Mice SCID 03 medical and health sciences chemistry.chemical_compound Mice In vivo Osteogenesis Animals Induced pluripotent stem cell General Dentistry Cells Cultured biology Cell Differentiation Mesenchymal Stem Cells Molecular biology In vitro Staining PLGA 030104 developmental biology chemistry Cell culture Ceramics and Composites Osteocalcin biology.protein Type I collagen Biomedical engineering |
| Zdroj: | Dental materials journal. 36(1) |
| ISSN: | 1881-1361 |
| Popis: | In the present report, we have generated osteoblast-like cells derived from mouse induced-pluripotent stem (iPS) cells on PLGA with osteoinduction medium in vitro and in vivo. The cell culture period was 2 weeks. At 2 weeks, mRNA level of type I collagen was significantly higher than at 1 week. Osteocalcin mRNA level at 2 weeks was tendency to increase compared with at 1 week. And the cells cultured on PLGA were positive for immunofluorescent staining of osteocalcin and alizarin red S staining. The scaffold and osteogenic-like cells induced in vitro were implanted subcutaneously into SCID mice. In resected teratoma, hard tissues resembling bone were observed mixed with other tissues on the scaffold. The sum of these findings suggests that PLGA does not disturb the osteogenesis of iPS cells. |
| Databáze: | OpenAIRE |
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