Kynurenine plays an immunosuppressive role in 2,4,6-trinitrobenzene sulfate-induced colitis in mice
| Autor: | Kentaro Nakamoto, Yasuko Yamamoto, Hiroyuki Tomita, Masato Hoshi, Tatsuya Ando, Hiroyuki Tezuka, Kuniaki Saito, Akira Hara, Chieko Tashita, Takayuki Hattori, Akihiro Hirata |
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| Rok vydání: | 2020 |
| Předmět: |
CD4-Positive T-Lymphocytes
Male Colon Regulatory T cell T cell Inflammatory bowel disease Mice 03 medical and health sciences chemistry.chemical_compound Kynurenine 3-monooxygenase 0302 clinical medicine Immune system Inflammatory bowie diseases medicine Animals Homeostasis Colitis Kynurenine Sulfates Chemistry Tryptophan Gastroenterology FOXP3 General Medicine Basic Study medicine.disease Ulcerative colitis digestive system diseases Mice Inbred C57BL Disease Models Animal medicine.anatomical_structure Trinitrobenzenes 030220 oncology & carcinogenesis Immunology 030211 gastroenterology & hepatology Immunosuppressive Agents |
| Zdroj: | World Journal of Gastroenterology |
| ISSN: | 1007-9327 |
| DOI: | 10.3748/wjg.v26.i9.918 |
| Popis: | Background Inflammatory bowel disease, such as Crohn's disease and ulcerative colitis, is characterized by chronic intestinal inflammation leading to intestinal mucosal damage. Inflammatory bowel disease causes dysregulation of mucosal T cell responses, especially the responses of CD4+ T cells. Previously, we demonstrated that indoleamine-2,3-dioxygenase plays an immunosuppressive role in 2,4,6-trinitrobenzene sulfate (TNBS)-induced colitis. Although indoleamine-2,3-dioxygenase exerts immunosuppressive effects by altering the local concentration of tryptophan (Trp) and immunomodulatory Trp metabolites, the specific changes in immune regulation during colitis caused by Trp metabolites and its related enzymes remain unclear. Aim To investigate role of kynurenine 3-monooxygenase (KMO) in TNBS-induced colitis and involvement of Trp metabolites in maintenance of intestinal homeostasis. Methods Colitis was induced in eight-week-old male KMO+/+ or KMO-/- mice of C57BL/6N background using TNBS. Three days later, the colon was used for hematoxylin-eosin staining for histological grading, immunohistochemical or immunofluorescence staining for KMO, cytokines, and immune cells. Inflammatory and anti-inflammatory cytokines were measured using quantitative RT-PCR, and kynurenine (Kyn) pathway metabolites were measured by high-performance liquid chromatography. The cell proportions of colonic lamina propria and mesenteric lymph nodes were analyzed by flow cytometry. Results KMO expression levels in the colonic mononuclear phagocytes, including dendritic cells and macrophages increased upon TNBS induction. Notably, KMO deficiency reduced TNBS-induced colitis, resulting in an increased frequency of Foxp3+ regulatory T cells and increased mRNA and protein levels of anti-inflammatory cytokines, including transforming growth factor-β and interleukin-10. Conclusion Absence of KMO reduced TNBS-induced colitis via generation of Foxp3+ regulatory T cells by producing Kyn. Thus, Kyn may play a therapeutic role in colon protection during colitis. |
| Databáze: | OpenAIRE |
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