Real-time imaging of apoptotic cell-membrane changes at the single-cell level in the beating murine heart

Autor:	Chris P. M. Reutelingsperger, Ewald A. W. J. Dumont, Mat J.A.P. Daemen, Pieter A. Doevendans, Leonard Hofstra, Jos F.M. Smits, Hein J.J. Wellens
Přispěvatelé:	Other departments
Rok vydání:	2001
Předmět:	Programmed cell death Real time imaging General Medicine Phosphatidylserine Plasma protein binding Biology Cellular level General Biochemistry Genetics and Molecular Biology Cell biology Cell membrane chemistry.chemical_compound medicine.anatomical_structure chemistry Annexin Apoptosis medicine
Zdroj:	Nature medicine, 7(12), 1352-1355. Nature Publishing Group
ISSN:	1546-170X 1078-8956
DOI:	10.1038/nm1201-1352
Popis:	We report a novel real-time imaging model to visualize apoptotic membrane changes of single cardiomyocytes in the injured heart of the living mouse, using fluorescent labeled annexin-V. Annexin-V binds to externalized phosphatidylserine (PS) of cells undergoing programmed cell death. With high-magnification (x100-160) real-time imaging, we visualized the binding of annexin-V to single cardiomyocytes. Kinetic studies at the single-cell level revealed that cardiomyocytes started to bind annexin-V within minutes after reperfusion, following an ischemic period of 30 minutes. The amount of bound annexin-V increased rapidly and reached a maximum within 20-25 minutes. Caspase inhibitors decreased the number of annexin-V-positive cardiomyocytes and slowed down the rate of PS exposure of cardiomyocytes that still bound annexin-V. This technology to study cell biology in the natural environment will enhance knowledge of intracellular signaling pathways relevant for cell-death regulation and strategies to manipulate these pathways for therapeutic effect.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0b09d9d1edcb141562c4f099b9c1ac0a https://doi.org/10.1038/nm1201-1352 Zobrazit plný text záznamu Plný text ve formátu PDF