Autophagy is a new protective mechanism against the cytotoxicity of platinum nanoparticles in human trophoblasts
| Autor: | Akemi Ushijima, Tae Kusabiraki, Tomoko Shima, Sayaka Tsuda, Yosuke Ono, Yasuo Tsutsumi, Yasuo Yoshioka, Shigeru Saito, Aiko Aoki, Akitoshi Nakashima, Kazuya Nagano, Kazuma Higashisaka, Osamu Yoshino |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cytoplasm DNA damage lcsh:Medicine Autophagy-Related Proteins Metal Nanoparticles Vascular Remodeling Article Cell Line 03 medical and health sciences 0302 clinical medicine Cell Movement Pregnancy Placenta Autophagy medicine Humans Particle Size lcsh:Science Cytotoxicity Cell Proliferation Platinum Cell Nucleus Multidisciplinary Chemistry Cell growth lcsh:R Trophoblasts Cell biology 030104 developmental biology medicine.anatomical_structure Cell culture lcsh:Q Female 030217 neurology & neurosurgery Homeostasis |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-13 (2019) |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-019-41927-2 |
| Popis: | Nanoparticles are widely used in commodities, and pregnant women are inevitably exposed to these particles. The placenta protects the growing fetus from foreign or toxic materials, and provides energy and oxygen. Here we report that autophagy, a cellular mechanism to maintain homeostasis, engulfs platinum nanoparticles (nPt) to reduce their cytotoxicity in trophoblasts. Autophagy was activated by nPt in extravillous trophoblast (EVT) cell lines, and EVT functions, such as invasion and vascular remodeling, and proliferation were inhibited by nPt. These inhibitory effects by nPt were augmented in autophagy-deficient cells. Regarding the dynamic state of nPt, analysis using ICP-MS demonstrated a higher accumulation of nPt in the autophagosome-rich than the cytoplasmic fraction in autophagy-normal cells. Meanwhile, there were more nPt in the nuclei of autophagy-deficient cells, resulting in greater DNA damage at a lower concentration of nPt. Thus, we found a new protective mechanism against the cytotoxicity of nPt in human trophoblasts. |
| Databáze: | OpenAIRE |
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