Regulation of dendritic cell differentiation and subset distribution by the zinc finger protein CTCF
Autor: | Christina Koesters, Ivan Bilic, Stefan Bluml, Wilfried Ellmeier, Martin Schreiber, Beate M. Lichtenberger, Bernd Unger, Uwe Schmidt, Johannes Stöckl |
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Rok vydání: | 2007 |
Předmět: |
CCCTC-Binding Factor
Cell Survival Cellular differentiation Immunology Regulator Down-Regulation Bone Marrow Cells Cell Growth Processes Dendritic cell differentiation Biology Mice Downregulation and upregulation Animals Humans Immunology and Allergy Transcription factor Zinc finger Mice Inbred BALB C Toll-Like Receptors Cell Differentiation Zinc Fingers Dendritic Cells Phenotype Molecular biology Cell biology DNA-Binding Proteins Mice Inbred C57BL Repressor Proteins CTCF Transcription Factors |
Zdroj: | Immunology Letters. 109:165-174 |
ISSN: | 0165-2478 |
DOI: | 10.1016/j.imlet.2007.02.006 |
Popis: | The molecular mechanisms that regulate DC differentiation and subset distribution are largely unknown. In this study we report the identification of the C(2)H(2) zinc finger transcription factors (TF) CTCF as a regulator of DC differentiation. CTCF was expressed in human and murine DC and its expression was downregulated during the differentiation of human monocyte-derived DC. Enforced expression of CTCF during the differentiation of murine BM-derived DC (BMDC) caused increased apoptosis and reduced proliferation leading to a dramatically reduced number of CTCF transduced DC. The CTCF expressing BMDC that developed had a more immature phenotype than control cells, and showed defects in maturation upon TLR stimulation. Furthermore, in vivo expression of CTCF led to an increase in the percentage of plasmacytoid DC (pDC) within the DC lineage. Our data provide new insight into molecular mechanisms regulating DC differentiation and subset development and identify CTCF as a factor involved in the regulation of these important processes. |
Databáze: | OpenAIRE |
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