Characterization of metastasis formation and virotherapy in the human C33A cervical cancer model

Autor: Stephanie Weibel, Jochen Stritzker, Johanna Langbein-Laugwitz, Christina Kober, Richard J. Aguilar, Juliane Rother, Ulrike Donat, Michael Hess, Aladar A. Szalay, Nanhai G. Chen, Barbara Härtl, Simon Schäfer
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Pathology
Tumor Physiology
Cancer Treatment
Uterine Cervical Neoplasms
lcsh:Medicine
Cervical Cancer
Metastasis
Hematologic Cancers and Related Disorders
Mice
Basic Cancer Research
Medicine and Health Sciences
Medicine
Neoplasm Metastasis
lcsh:Science
ddc:616
Cervical cancer
Oncolytic Virotherapy
Multidisciplinary
Cell Cycle
Hematology
Animal Models
Lymphatic system
Oncology
Research Design
Lymphatic Metastasis
Female
Lymphomas
Research Article
medicine.medical_specialty
Clinical Research Design
Mouse Models
Research and Analysis Methods
Models
Biological
Model Organisms
Cell Line
Tumor
Animals
Humans
Animal Models of Disease
Virotherapy
Renal Lymph Node
business.industry
fungi
lcsh:R
Cancer
Biology and Life Sciences
Cancers and Neoplasms
medicine.disease
Oncolytic virus
Microscopy
Fluorescence
Cancer cell
Animal Studies
lcsh:Q
business
Gynecological Tumors
Zdroj: PLoS ONE, Vol 9, Iss 6, p e98533 (2014)
PLoS ONE
ISSN: 1932-6203
Popis: More than 90% of cancer mortalities are due to cancer that has metastasized. Therefore, it is crucial to intensify research on metastasis formation and therapy. Here, we describe for the first time the metastasizing ability of the human cervical cancer cell line C33A in athymic nude mice after subcutaneous implantation of tumor cells. In this model, we demonstrated a steady progression of lumbar and renal lymph node metastases during tumor development. Besides predominantly occurring lymphatic metastases, we visualized the formation of hematogenous metastases utilizing red fluorescent protein (RFP) expressing C33A-RFP cells. RFP positive cancer cells were found migrating in blood vessels and forming micrometastases in lungs of tumor-bearing mice. Next, we set out to analyze the influence of oncolytic virotherapy in the C33A-RFP model and demonstrated an efficient virus-mediated reduction of tumor size and metastatic burden. These results suggest the C33A-RFP cervical cancer model as a new platform to analyze cancer metastases as well as to test novel treatment options to combat metastases.
Databáze: OpenAIRE
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