Identification of Key Structural Motifs Involved in 7 Transmembrane Signaling of Adhesion GPCRs
| Autor: | Rob Leurs, Henry F. Vischer, Marta Arimont, Chris de Graaf, Melanie van der Woude, Saskia Nijmeijer |
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| Přispěvatelé: | Medicinal chemistry, AIMMS |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Pharmacology
ADGRG1 Mutagenesis (molecular biology technique) Computational biology Adhesion Biology adhesion GPCR Transmembrane protein ELTD1 GPR56 SDG 3 - Good Health and Well-being class B2 Pharmacology (medical) Identification (biology) Sequence motif Structural motif ADGRL4 G protein-coupled receptor |
| Zdroj: | Arimont, M, Van Der Woude, M, Leurs, R, Vischer, H F, De Graaf, C & Nijmeijer, S 2019, ' Identification of Key Structural Motifs Involved in 7 Transmembrane Signaling of Adhesion GPCRs ', ACS Pharmacology & Translational Science, vol. 2, no. 2, pp. 101-113 . https://doi.org/10.1021/acsptsci.8b00051 ACS Pharmacology & Translational Science, 2(2), 101-113. American Chemical Society |
| ISSN: | 2575-9108 |
| Popis: | The adhesion class B2 family of G protein-coupled receptors (GPCRs) plays a key role in important physiological processes and their dysfunction is linked to brain malformations and tumorigenesis. Although information regarding their signaling properties is starting to emerge, the structural motifs and interaction networks that determine 7 transmembrane (TM) signaling of class B2 GPCRs remain to be elucidated. Comparative sequence-structure analyses of class B2 GPCRs and the recently solved active class B1 structures show that class B2 GPCRs include different elements of the conserved residue motifs that determine class B1 activation. Combined site-directed mutagenesis and molecular dynamics studies were performed to give detailed insights into the role of 7TM interaction networks in signaling of two representative class B2 receptors, ADGRG1 (GPR56) and ADGRL4 (ELTD1). The systematic investigation of class B1/B2 sequence motifs provides consistent structure-function relationships that can be translated to the whole class B2 GPCR family and suggests that class B1 and B2 GPCRs share conserved intramolecular 7TM interactions. This improved understanding in adhesion GPCR structure and constitutive signaling can accelerate drug design campaigns for this chemically unexplored receptor class. |
| Databáze: | OpenAIRE |
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