A prospective, proteomics study identified potential biomarkers of encapsulating peritoneal sclerosis in peritoneal effluent

Autor: Timothy S. Johnson, Caroline A. Evans, Martin Wilkie, Vasileios Zavvos, Paul Brenchley, Dimitrios S. Goumenos, Simon J. Davies, Angela Summers, Nicholas Topley, Mark Lambie, Anthony T. Buxton
Rok vydání: 2016
Předmět:
0301 basic medicine
Adult
Male
Proteomics
Pathology
medicine.medical_specialty
Peritoneum/pathology
Apolipoprotein B
Proteome
Peritoneal Dialysis/adverse effects
medicine.medical_treatment
Dermatopontin
Fibrinogen
Biomarkers/analysis
Risk Assessment
Peritoneal dialysis
03 medical and health sciences
Dialysis Solutions
medicine
Proteome/analysis
Dialysis Solutions/chemistry
Humans
Prospective Studies
Peritoneal Fibrosis/diagnosis
Prospective cohort study
Risk Assessment/methods
Aged
biology
business.industry
Peritoneal Fibrosis
Middle Aged
Prognosis
R1
030104 developmental biology
Kidney Failure
Chronic/therapy
Nephrology
biology.protein
Kidney Failure
Chronic
Electrophoresis
Polyacrylamide Gel
Female
Proteomics/methods
Peritoneum
Complication
business
Retinol binding
Peritoneal Dialysis
Biomarkers
medicine.drug
Zdroj: Zavvos, V, Buxton, A T, Evans, C, Lambie, M, Davies, S J, Topley, N, Wilkie, M, Summers, A, Brenchley, P, Goumenos, D S & Johnson, T S 2017, ' A prospective, proteomics study identified potential biomarkers of encapsulating peritoneal sclerosis in peritoneal effluent ', Kidney International, vol. 92, no. 4, pp. 988-1002 . https://doi.org/10.1016/j.kint.2017.03.030
ISSN: 1523-1755
0085-2538
DOI: 10.1016/j.kint.2017.03.030
Popis: Encapsulating peritoneal sclerosis (EPS) is a potentially devastating complication of peritoneal dialysis\ud (PD). Diagnosis is often delayed due to the lack of effective and accurate diagnostic tools. We\ud therefore examined peritoneal effluent for potential biomarkers that could predict or confirm the\ud diagnosis of EPS and would be valuable in stratifying at-risk patients, and driving appropriate\ud interventions. Using prospectively collected samples from the Global Fluid Study and a cohort of\ud Greek PD patients, we utilized 2D SDSPAGE/ MS and iTRAQ to identify changes in the peritoneal\ud effluent proteome from patients diagnosed with EPS and controls matched for treatment exposure. We\ud employed a combinatorial peptide ligand library to compress the dynamic range of protein\ud concentrations, to aid identification of low-abundance proteins. In patients with stable membrane\ud function, fibrinogen γ-chain and heparan sulphate proteoglycan core protein progressively\ud increased over time on PD. In patients who developed EPS, collagen-α1(I), γ-actin and Complement\ud factors B and I were elevated up to five years prior to diagnosis. Orosomucoid-1 and a2-HSglycoprotein chain-B were elevated about one year before diagnosis, while apolipoprotein A-IV and\ud α1-antitrypsin were decreased compared to controls. Dynamic range compression resulted in an\ud increased number of proteins detected with improved resolution of protein spots, compared to the full\ud fluid proteome. Intelectin-1, dermatopontin, gelsolin and retinol binding protein-4 were elevated in\ud proteome-mined samples from patients with EPS compared to patients that had just commenced\ud peritoneal dialysis. Thus, prospective analysis of peritoneal effluent uncovered proteins indicative of\ud inflammatory and pro-fibrotic injury worthy of further evaluation as diagnostic/prognostic markers.
Databáze: OpenAIRE
Externí odkaz: