Protein disulfide isomerase A3 might be involved in the regulation of 24-dehydrocholesterol reductase via vitamin D equilibrium in primary cortical neurons
| Autor: | Spyridon N. Karras, Duygu Gezen-Ak, Erdinç Dursun, Merve Alaylıoğlu, Büşra Şengül, Ulaş Yavuz |
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| Rok vydání: | 2021 |
| Předmět: |
Oxidoreductases Acting on CH-CH Group Donors
medicine.medical_specialty Protein Disulfide-Isomerases Neocortex Nerve Tissue Proteins PDIA3 Calcitriol receptor Rats Sprague-Dawley 7-Dehydrocholesterol chemistry.chemical_compound Calcitriol Internal medicine medicine Vitamin D and neurology Animals Gene Silencing RNA Small Interfering Protein disulfide-isomerase Cells Cultured Neurons Messenger RNA Chemistry Endoplasmic reticulum Neurodegeneration Cell Biology General Medicine medicine.disease Endocrinology Gene Expression Regulation Receptors Calcitriol Developmental Biology |
| Zdroj: | In Vitro Cellular & Developmental Biology - Animal. 57:704-714 |
| ISSN: | 1543-706X 1071-2690 |
| DOI: | 10.1007/s11626-021-00602-5 |
| Popis: | Vitamin D is a secosteroid hormone mediating its functions via vitamin D receptor (VDR) and an endoplasmic reticulum chaperone, protein disulfide isomerase A3 (PDIA3). From a physiological perspective, there is also a well-established association of cholesterol and vitamin D synthesis, since both share a common metabolic substrate, 7 dehydrocholesterol (7-DHC). Yet, the potential basic pathways, of the biological interplay of DHCR24 and vitamin D equilibrium, on neuronal level, are yet to be determined. In this study, we aimed to investigate the relation between vitamin D pathways and DHCR24 in primary cortical neuron cultures. The neocortex of Sprague-Dawley rat embryos (E16) was used for the preparation of primary cortical neuron cultures. DHCR24 mRNA and protein expression levels were determined by qRT-PCR, Western blotting, and immunofluorescent labeling in 1,25-dihydroxyvitamin D3-treated or VDR/PDIA3-silenced primary cortical neurons. The mRNA expression of DHCR24 was significantly decreased in the cortical neurons treated with 10(-8)M 1,25-dihydroxyvitamin D-3 (p |
| Databáze: | OpenAIRE |
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