Chemiluminescent biosensors based on porous supports with immobilized peroxidase
| Autor: | Galina V. Kovba, Alexey M. Egorov, Maya Yu. Rubtsova |
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| Rok vydání: | 1998 |
| Předmět: |
Immobilized enzyme
Biomedical Engineering Biophysics Biosensing Techniques Horseradish peroxidase Sensitivity and Specificity law.invention chemistry.chemical_compound Mice law Electrochemistry Animals Hydrogen peroxide Chemiluminescence Peroxidase Detection limit Immunoassay Chromatography biology Herbicides General Medicine Hydrogen Peroxide Enzymes Immobilized chemistry Luminescent Measurements biology.protein 2 4-Dichlorophenoxyacetic Acid Biosensor Nitrocellulose Biotechnology |
| Zdroj: | Biosensorsbioelectronics. 13(1) |
| ISSN: | 0956-5663 |
| Popis: | Combining a sensitive and fast chemiluminescence detection method with novel immobilization methods for horseradish peroxidase (HRP) has enabled us to develop sensitive sensors for hydrogen peroxide and the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). HRP biospecific immobilization was shown to have well-defined advantages in terms of a higher specific activity of the enzyme on the surface and a greater sensitivity in the enhanced chemiluminescence (ECL) reaction. The lower detection limit for hydrogen peroxide was 0·025 nmol with HRP immobilized on nitrocellulose membrane through avidin-biotin linkage. An immunosensor for the detection of 2,4-D provides a lower detection limit of 0·2 μg/l with specific antibodies covalently immobilized on photoactivated nylon. The assay with chemiluminescent detection was also characterized with a wider concentration range when compared with the colorimetric assay. |
| Databáze: | OpenAIRE |
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