VIP receptors and control of short circuit current in the human intestinal clonal cell line Cl.19A
| Autor: | Estelle Grasset, Christian L. Laboisse, Chantal Augeron, Marc Laburthe, Christiane Rouyer-Fessard, Jean-José Maoret |
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| Rok vydání: | 1989 |
| Předmět: |
Adenylate kinase
Stimulation Biology Adenocarcinoma Binding Competitive Epithelium Receptors Gastrointestinal Hormone Cellular and Molecular Neuroscience Tumor Cells Cultured Humans Receptor Molecular Biology Pharmacology Binding protein Electric Conductivity Cell Biology Dissociation constant Intestines Molecular Weight Biochemistry Membrane protein Cell culture Colonic Neoplasms Biophysics Molecular Medicine Receptors Vasoactive Intestinal Peptide Cyclase activity hormones hormone substitutes and hormone antagonists Adenylyl Cyclases Vasoactive Intestinal Peptide |
| Zdroj: | Experientia. 45(11-12) |
| ISSN: | 0014-4754 |
| Popis: | At the maximally effective concentration of 10 nM, VIP induced a marked (12.5-fold stimulation above basal), and sustained increase in short circuit current in the human intestinal epithelial cell line Cl.19A grown on permeable filters and placed in Ussing chambers. Half-maximal increase of Isc was observed for 0.1 nM VIP. This was well correlated with the VIP-stimulated adenylate cyclase activity (ED50:0.07 nM). Binding studies using 125I-VIP indicated that Cl.19A cells express a peptide-specific VIP receptor with a dissociation constant of 0.07 nM. Covalent labeling of receptors followed by SDS-PAGE analysis of membrane proteins resulted in the identification of a 63,000 dalton binding protein in Cl.19A cells. |
| Databáze: | OpenAIRE |
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