Simultaneous in vivo time-lapse stiffness mapping and fluorescence imaging of developing tissue
| Autor: | Kristian Franze, Eva K Pillai, Ivan B Dimov, Amelia J Thompson, Christine E. Holt |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0303 health sciences
Fluorescence-lifetime imaging microscopy biology Chemistry Dynamics (mechanics) Xenopus Stiffness Embryonic Tissue biology.organism_classification 03 medical and health sciences 0302 clinical medicine In vivo medicine Biophysics Tissue mechanics Tissue stiffness medicine.symptom 030217 neurology & neurosurgery 030304 developmental biology |
| DOI: | 10.1101/323501 |
| Popis: | Tissue mechanics is important for development; however, the spatio-temporal dynamics of in vivo tissue stiffness is still poorly understood. We here developed tiv-AFM, combining time-lapse in vivo atomic force microscopy with upright fluorescence imaging of embryonic tissue, to show that in the developing Xenopus brain, a stiffness gradient evolves over time because of differential cell proliferation. Subsequently, axons turn to follow this gradient, underpinning the importance of time-resolved mechanics measurements. |
| Databáze: | OpenAIRE |
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