Molecular and biochemical characterisation ofMycobacterium smegmatisalcohol dehydrogenase C
| Autor: | Alexandra Galamba, Karine Soetaert, Paul Buyssens, Didier Monnaie, Paul Jacobs, Jean Content |
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| Rok vydání: | 2001 |
| Předmět: |
Molecular Sequence Data
Mycobacterium smegmatis Dehydrogenase Microbiology Mycobacterium Substrate Specificity Mycobacterium tuberculosis Genetics Amino Acid Sequence Cloning Molecular Molecular Biology Aldehyde Reductase Alcohol dehydrogenase chemistry.chemical_classification Aldehydes Mycobacterium bovis Base Sequence biology Alcohol Dehydrogenase biology.organism_classification Enzyme chemistry Biochemistry Genes Bacterial biology.protein Sequence Alignment |
| Zdroj: | FEMS Microbiology Letters. 196:51-56 |
| ISSN: | 1574-6968 0378-1097 |
| DOI: | 10.1111/j.1574-6968.2001.tb10539.x |
| Popis: | The gene encoding of an alcohol dehydrogenase C (ADHC) from Mycobacterium smegmatis was cloned and sequenced. The protein encoded by this gene has 78% identity with Mycobacterium tuberculosis and Mycobacterium bovis BCG ADHC. The M. smegmatis ADHC was purified from M. smegmatis and the kinetic parameters of this enzyme showed that using NADPH as electron donor it has a strong preference for aliphatic and aromatic aldehyde substrates. Like the M. bovis BCG ADHC, this enzyme is more likely to act as an aldehyde reductase than as an alcohol dehydrogenase. The discovery of such an ADHC in a fast-growing, and easily engineered mycobacterial species opens the way to the utilisation of this M. smegmatis enzyme as a convenient model for the study of the physiological role of this alcohol dehydrogenase in mycobacteria. |
| Databáze: | OpenAIRE |
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