Effects of isoflurane on auditory evoked potentials in the cochlea and brainstem of guinea pigs
| Autor: | Mark C. J. Aarts, H. Christiaan Stronks, Sjaak F.L. Klis |
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| Rok vydání: | 2010 |
| Předmět: |
Time Factors
Guinea Pigs Fluanisone Fentanyl Evoked Potentials Auditory Brain Stem Reaction Time medicine Animals Auditory system Wakefulness Dose-Response Relationship Drug Isoflurane Chemistry Sensory Systems Cochlea Compound muscle action potential Electrophysiology medicine.anatomical_structure Auditory brainstem response Acoustic Stimulation Anesthesia Anesthetics Inhalation Anesthetic Cochlear Microphonic Potentials Female Brain Stem medicine.drug |
| Zdroj: | Hearing Research. 260:20-29 |
| ISSN: | 0378-5955 |
| Popis: | Electrophysiological recordings of the auditory system are commonly performed in deeply anesthetized animals. This study evaluated the effects of various concentrations of the volatile anesthetic isoflurane (1-3%) on the compound action potential (CAP), cochlear microphonic (CM) and auditory brainstem response (ABR). Recordings were initiated in the awake, lightly restrained animal. Anesthesia was induced with a single dose of Hypnorm (fentanyl and fluanisone). After tracheostomy increasing isoflurane concentrations were applied in N(2)O/O(2) via controlled ventilation. Data were compared to recordings in the awake animal using repeated measures ANOVA and Dunnett's post hoc test. On average, isoflurane dose-dependently suppressed the amplitude and increased the latency of the CAP. CM amplitude was suppressed. These effects were most profound at high frequencies and were typically significant at isoflurane concentrations of 2.5% and 3%. Amplitude and latency of the second negative peak of the CAP (N(2)) were affected to a greater extent compared to the first peak (N(1)). On average, isoflurane dose-dependently reduced the amplitude and increased the latency of the ABR. These effects were typically significant at an isoflurane concentration of 2%. Effects on peak IV and V were more pronounced compared to the early peaks I and III. |
| Databáze: | OpenAIRE |
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